Document Detail

Corticosteroid effect on Caco-2 cell lipids depends on cell differentiation.
MedLine Citation:
PMID:  14672736     Owner:  NLM     Status:  MEDLINE    
Previous studies from our laboratory have indicated that secondary hyperaldosteronism affects phospholipids of rat colonic enterocytes. To assess whether this represents a direct effect of mineralocorticoids on enterocytes, the role of aldosterone and dexamethasone in the regulation of lipid metabolism was examined in Caco-2 cells during development of their enterocyte phenotype. Differentiation of Caco-2 cells was associated with increased levels of triglycerides (TG) and cholesteryl esters (CE), a decreased content of cholesterol and phospholipids and changes in individual phospholipid classes. The phospholipids of differentiated cells had a higher content of n-6 polyunsaturated fatty acids (PUFA) and lower amounts of monounsaturated (MUFA) and saturated fatty acids than subconfluent undifferentiated cells. Differentiated cells exhibited a higher ability to incorporate [3H]arachidonic acid (AA) into cellular phospholipids and a lower ability for incorporation into TG and CE. Incubation of subconfluent undifferentiated cells with aldosterone or dexamethasone was without effect on the content of lipids, their fatty acids and [3H]AA incorporation. In contrast, aldosterone treatment of differentiated cells diminished the content of TG, increased the content of phospholipids and modulated their fatty acid composition. The percentage of n-6 and n-3 PUFA in phospholipids was increased and that of MUFA decreased, whereas no changes in TG were observed. The incorporation of [3H]AA into phospholipids was increased and into TG decreased and these changes were blocked by spironolactone. Treatment of differentiated cells with dexamethasone increased their CE content but no effect was identified upon other lipids, their fatty acid composition and on the incorporation of [3H]AA. As expected for the involvement of corticosteroid hormones the mineralocorticoid and glucocorticoid receptors were identified in Caco-2 cells by RT-PCR. The results suggest that aldosterone had a profound influence on lipid metabolism in enterocytes and that its effect depends on the stage of differentiation. The aldosterone-dependent changes occurring in phospholipids and their fatty acid composition may reflect a physiologically important phenomenon with long-term consequences for membrane structure and function.
S Jindrichová; O Nováková; J Bryndová; E Tvrzická; V Lisá; F Novák; J Pácha
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of steroid biochemistry and molecular biology     Volume:  87     ISSN:  0960-0760     ISO Abbreviation:  J. Steroid Biochem. Mol. Biol.     Publication Date:  2003 Nov 
Date Detail:
Created Date:  2003-12-15     Completed Date:  2004-02-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9015483     Medline TA:  J Steroid Biochem Mol Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  157-65     Citation Subset:  IM    
Institute of Physiology, Czech Academy of Sciences, Vídenská 1083, 142 20 Prague 4-Krc, Czech Republic.
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MeSH Terms
Aldosterone / metabolism,  pharmacology*
Arachidonic Acid / metabolism
Caco-2 Cells
Cell Differentiation / physiology*
Cholesterol / metabolism
Dexamethasone / pharmacology*
Enterocytes / cytology,  drug effects*,  metabolism*
Fatty Acids / metabolism
Lipid Metabolism*
Phospholipids / metabolism
RNA, Messenger / analysis,  biosynthesis
Receptors, Glucocorticoid / biosynthesis
Receptors, Mineralocorticoid / biosynthesis
Spironolactone / pharmacology
Triglycerides / metabolism
Reg. No./Substance:
0/Fatty Acids; 0/Phospholipids; 0/RNA, Messenger; 0/Receptors, Glucocorticoid; 0/Receptors, Mineralocorticoid; 0/Triglycerides; 10028-17-8/Tritium; 50-02-2/Dexamethasone; 506-32-1/Arachidonic Acid; 52-01-7/Spironolactone; 52-39-1/Aldosterone; 57-88-5/Cholesterol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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