Document Detail


Corticosteroid-binding globulin status at the fetomaternal interface during human term pregnancy.
MedLine Citation:
PMID:  11207196     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The status of the corticosteroid-binding globulin (CBG) at the fetomaternal interface, especially in the maternal intervillous blood space (I), was investigated and compared to that of CBG in the maternal (M) and fetal (umbilical arteries [A] and vein [V]) peripheral circulations at term. Immunoquantitation of plasma CBG showed that the CBG concentration in I was 30% less than that in M (P < 0.001) and threefold higher than that in umbilical cord blood (P < 0.001). The microheterogeneity of CBG studied by immunoaffinoelectrophoresis in the presence of concanavalin A and Western blotting indicated that the CBG in I was mainly of maternal origin and different from fetal CBG. A CBG mRNA, but no classic 50- to 59-kDa CBG, was found in isolated term trophoblastic cells. The steroid environment of the CBG in I differed greatly from that in the peripheral maternal and fetal circulations, because the progesterone:cortisol molar ratio in I was 75-fold higher than that in M and 7- to 10-fold higher than that in the fetal circulation. Binding studies revealed that the affinity constants of CBG for cortisol in I, A, and V were significantly lower than that in M plasma (P < 0.02) in their respective hormonal contexts. The binding parameters for I-CBG stripped of endogenous steroids and lipids were close to those for M-CBG but different from those of fetal CBG (P < 0.001). These data reflect the physiological relevance of the CBG-steroid interaction, especially with very CBG-loaded progesterone at the fetomaternal interface during late pregnancy.
Authors:
C Benassayag; I Souski; T M Mignot; B Robert; J Hassid; P Duc-Goiran; F Mondon; R Rebourcet; L Dehennin; E A Nunez; F Ferré
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biology of reproduction     Volume:  64     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2001 Mar 
Date Detail:
Created Date:  2001-03-14     Completed Date:  2001-05-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  812-21     Citation Subset:  IM    
Affiliation:
INSERM U.361, Maternité Port-Royal Cochin, Université René Descartes, 75014 Paris, France. u361@cochin.inserm.fr
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MeSH Terms
Descriptor/Qualifier:
Adult
Blotting, Western
Cesarean Section
Enzyme-Linked Immunosorbent Assay
Female
Fetal Blood / metabolism*,  physiology
Gas Chromatography-Mass Spectrometry
Humans
Hydrocortisone / blood
Immunoelectrophoresis, Two-Dimensional
Immunomagnetic Separation
Male
Maternal-Fetal Exchange / physiology*
Placenta / metabolism*,  physiology
Pregnancy / blood,  metabolism*
Progesterone / blood
Protein Isoforms
Radioimmunoassay
Reverse Transcriptase Polymerase Chain Reaction
Serum Albumin / metabolism
Transcortin / metabolism*,  physiology
Trophoblasts / metabolism,  physiology
Chemical
Reg. No./Substance:
0/Protein Isoforms; 0/Serum Albumin; 50-23-7/Hydrocortisone; 57-83-0/Progesterone; 9010-38-2/Transcortin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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