| Corticoliberin-immunoreactive cell bodies localised in two distinct areas of the sheep hypothalamus. | |
| | |
MedLine Citation:
|
PMID: 6410305 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The localisation of corticoliberin producing neurones in the sheep hypothalamus was attempted with an antiserum directed against synthetic ovine CRF by the indirect immunofluorescence procedure. Synthetic ovine corticoliberin-immunoreactive fibres were detected, in order of decreasing importance, in the external median eminence, in the caudal neural lobe around capillaries, at the boundary of the neural and intermediate lobe, around the anterior commissure, in the paraventricular nuclei and in the posterior hypothalamus and midbrain, suggesting that synthetic ovine corticoliberin-related substances act not only on anterior pituitary tissue, but also on the intermediate lobe, on central neurones and on peripheral target organs. Two groups of cell bodies reacted to the anti-synthetic ovine corticoliberin antiserum. The first group was located in the paraventricular nuclei and consisted of 15-20 microns diameter cell bodies with a granular cytoplasm. The second group was located mainly in the dorsolateral caudal hypothalamus, and the cell bodies were smaller (10-15 microns) and had a smooth cytoplasm. No cell bodies were detected in the basal hypothalamus). Synthetic ovine corticoliberin-immunoreactive structures did not contain immunoreactive neurophysin. The synthetic ovine corticoliberin-immunoreaction in the paraventricular neurones was abolished by preincubating the antiserum with synthetic ovine corticoliberin but not with sauvagine or several other peptides. The immunoreaction in the posterior hypothalamic groups was abolished by preincubating the synthetic ovine corticoliberin antiserum with both synthetic ovine corticoliberin and sauvagine, but not with other peptides. The results suggest that the immunoreaction was specific for synthetic ovine corticoliberin in the paraventricular but not posterior hypothalamic region. The relative contribution of both areas to synthetic ovine corticoliberin-like peptides containing nerve terminals of the median eminence remains to be established. |
| | |
Authors:
|
E Kolodziejczyk; A J Baertschi; G Tramu |
Related Documents
:
|
6273145 - Species specificity of radioreceptor assay and radioimmunoassay for rat fsh. 6318885 - Endorphin-related peptides in rat cerebrospinal fluid. 2509615 - Growth hormone-releasing factor-induced growth hormone secretion from perifused rat ant... 6811685 - Superovulation induced by a single pituitary gland transplanted beneath the kidney caps... 10571475 - The hippocampus is not necessary for a place response but may be necessary for pliancy. 7026195 - Dexamethasone hypertension in rats. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Neuroscience Volume: 9 ISSN: 0306-4522 ISO Abbreviation: Neuroscience Publication Date: 1983 Jun |
Date Detail:
|
Created Date: 1983-09-23 Completed Date: 1983-09-23 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 7605074 Medline TA: Neuroscience Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 261-70 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Corticotropin-Releasing Hormone / metabolism* Fluorescent Antibody Technique Hypothalamus / metabolism* Hypothalamus, Posterior / metabolism Mamillary Bodies / metabolism Median Eminence / metabolism Nerve Fibers / metabolism Neurons / metabolism Neurophysins / metabolism Paraventricular Hypothalamic Nucleus / metabolism Pituitary Gland, Posterior / metabolism Sheep |
| Chemical | |
Reg. No./Substance:
|
0/Neurophysins; 9015-71-8/Corticotropin-Releasing Hormone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Presence of octopamine in firefly photomotor neurons.
Next Document: Inhibition of norepinephrine autoxidation by a rat brain cortical factor.