Document Detail


Cortical granule matrix disassembly during exocytosis in sea urchin eggs.
MedLine Citation:
PMID:  1720747     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cortical granule exocytosis in sea urchins was studied using hyperosmotic and polymer-containing seawater to halt granule matrix dispersal. Addition of Na2SO4-containing seawater (2.5 osmole/kg) to Strongylocentrotus purpuratus eggs 10 to 40 sec after insemination resulted in arrest of the exocytic wave during propagation. EM examination of these eggs revealed that matrix disassembly occurred in distinct stages. In the earliest stage, granule-plasma membrane fusion had occurred, but the matrix remained completely intact. This early stage was observed in hyperosmotic media, either ionic or nonionic, suggesting that matrix hydration is required for disassembly and exocytic pore widening, but not for membrane fusion. Subsequent stages, in which partially disassembled matrices remained within omega-configured pockets, were captured by activating eggs in 30% dextran in seawater. Stability of these intermediates stages required the presence of Ca2+ and Mg2+; in the absence of divalent cations the matrices completely disassembled and the exocytic pockets flattened. Divalent cations appeared to prevent fragmentation of the matrix lamellae. Late stages of matrix disassembly, in which the lamellae fragmented and formed small particles, were inhibited by media of high ionic strength. Hyperosmolality alone, provided by sucrose, was unable to halt these late stages suggesting that water availability does not play an important role once a critical point in matrix dispersal has been reached.
Authors:
C J Merkle; D E Chandler
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Developmental biology     Volume:  148     ISSN:  0012-1606     ISO Abbreviation:  Dev. Biol.     Publication Date:  1991 Dec 
Date Detail:
Created Date:  1992-01-14     Completed Date:  1992-01-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  429-41     Citation Subset:  IM    
Affiliation:
Department of Zoology, Arizona State University, Tempe 85287-1501.
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MeSH Terms
Descriptor/Qualifier:
Animals
Calcium / pharmacology
Cell Membrane / ultrastructure
Cytoplasmic Granules / ultrastructure*
Dextrans / pharmacology
Exocytosis* / drug effects
Fertilization*
Freeze Fracturing
Magnesium / pharmacology
Microscopy, Electron
Osmolar Concentration
Ovum / physiology*,  ultrastructure
Sea Urchins / embryology*
Chemical
Reg. No./Substance:
7439-95-4/Magnesium; 7440-70-2/Calcium; 9004-54-0/Dextrans

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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