Document Detail


Cortical astrocytes activated by basic fibroblast growth factor secrete molecules that stimulate differentiation of mesencephalic dopaminergic neurons.
MedLine Citation:
PMID:  1279704     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In reactive gliosis, astrocytes undergo morphological and biochemical changes which can be mimicked in vitro by treatment with bFGF (basic fibroblast growth factor) or cAMP. To investigate the influence of activated cortical astrocytes on central nervous system (CNSD) neurons, we studied the effect of the supernatant from bFGF-treated astrocytes on the development of dopaminergic neurons from rat mesencephalon. Conditioned medium of untreated astrocytes stimulated dopamine uptake of mesencephalic cultures. After activation of astrocytes with bFGF this effect was greatly enhanced. It was significantly more potent than stimulating effects of other neurotrophic factors. The supernatant of these astrocytes increased the biochemical differentiation but not the survival of dopaminergic neurons in our cell culture system. Trypsin digestion and gel chromatography revealed that the activity was due to one or several proteins with molecular mass above 5 kDa. We excluded the participation of several factors known to be produced by astrocytes or that are neurotrophic for substantia nigra cultures. In particular, we provide evidence that bFGF, BDNF, NT-3, Il-1, Il-6, S100 beta and alpha 2-macroglobulin were not involved in the effect of the conditioned medium. In vitro stimulation of astrocytes therefore triggers the expression of currently uncharacterized factors which influence the biochemical differentiation of mesencephalic dopaminergic neurons, the cells that degenerate in Parkinson's disease.
Authors:
G Gaul; H Lübbert
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Proceedings. Biological sciences / The Royal Society     Volume:  249     ISSN:  0962-8452     ISO Abbreviation:  Proc. Biol. Sci.     Publication Date:  1992 Jul 
Date Detail:
Created Date:  1992-12-16     Completed Date:  1992-12-16     Revised Date:  2005-04-08    
Medline Journal Info:
Nlm Unique ID:  101245157     Medline TA:  Proc Biol Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  57-63     Citation Subset:  IM    
Affiliation:
Preclinical Research, Sandoz Pharma Ltd, Basel, Switzerland.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Astrocytes / drug effects,  physiology*,  secretion
Base Sequence
Brain-Derived Neurotrophic Factor
Cell Differentiation / physiology*
Cells, Cultured
Cerebral Cortex / physiology*
Culture Media
Dopamine / metabolism*
Fibroblast Growth Factor 2 / pharmacology*
Growth Substances / genetics,  pharmacology,  secretion*
Mesencephalon / cytology,  physiology*
Molecular Sequence Data
Nerve Growth Factors / genetics
Nerve Tissue Proteins / genetics*,  secretion
Neurons / cytology,  drug effects,  physiology*
Oligodeoxyribonucleotides
Polymerase Chain Reaction
RNA / genetics,  isolation & purification
RNA, Messenger / isolation & purification,  metabolism
Rats
Rats, Wistar
Recombinant Proteins / pharmacology
Tyrosine 3-Monooxygenase / metabolism*
Chemical
Reg. No./Substance:
0/Brain-Derived Neurotrophic Factor; 0/Culture Media; 0/Growth Substances; 0/Nerve Growth Factors; 0/Nerve Tissue Proteins; 0/Oligodeoxyribonucleotides; 0/RNA, Messenger; 0/Recombinant Proteins; 103107-01-3/Fibroblast Growth Factor 2; 63231-63-0/RNA; EC 1.14.16.2/Tyrosine 3-Monooxygenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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