| Cortactin mediated morphogenic cell movements during zebrafish (Danio rerio) gastrulation. | |
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MedLine Citation:
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PMID: 16483139 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cell migration is essential to direct embryonic cells to specific sites at which their developmental fates are ultimately determined. However, the mechanism by which cell motility is regulated in embryonic development is largely unknown. Cortactin, a filamentous actin binding protein, is an activator of Arp2/3 complex in the nucleation of actin cytoskeleton at the cell leading edge and acts directly on the machinery of cell motility. To determine whether cortactin and Arp2/3 mediated actin assembly plays a role in the morphogenic cell movements during the early development of zebrafish, we initiated a study of cortactin expression in zebrafish embryos at gastrulating stages when massive cell migrations occur. Western blot analysis using a cortactin specific monoclonal antibody demonstrated that cortactin protein is abundantly present in embryos at the most early developmental stages. Immunostaining of whole-mounted embryo showed that cortactin immunoreactivity was associated with the embryonic shield, predominantly at the dorsal side of the embryos during gastrulation. In addition, cortactin was detected in the convergent cells of the epiblast and hypoblast, and later in the central nervous system. Immunofluorescent staining with cortactin and Arp3 antibodies also revealed that cortactin and Arp2/3 complex colocalized at the periphery and many patches associated with the cell-to-cell junction in motile embryonic cells. Therefore, our data suggest that cortactin and Arp2/3 mediated actin polymerization is implicated in the cell movement during gastrulation and perhaps the development of the central neural system as well. |
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Authors:
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Dan Yu; Peijun Zhang; Xi Zhan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Science in China. Series C, Life sciences / Chinese Academy of Sciences Volume: 48 ISSN: 1006-9305 ISO Abbreviation: Sci. China, C, Life Sci. Publication Date: 2005 Dec |
Date Detail:
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Created Date: 2006-02-17 Completed Date: 2006-07-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9611809 Medline TA: Sci China C Life Sci Country: China |
Other Details:
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Languages: eng Pagination: 601-7 Citation Subset: IM |
Affiliation:
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Experimental Marine Biology Lab, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. Kathy@qing-daonews.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Western / methods Cell Movement* Central Nervous System / embryology Cortactin / biosynthesis*, genetics, physiology* Gastrula / cytology, physiology Gene Expression Regulation, Developmental* Sequence Homology, Amino Acid Zebrafish / embryology* |
| Chemical | |
Reg. No./Substance:
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0/Cortactin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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