Document Detail


Cortactin is involved in transforming growth factor-beta1-induced epithelial-mesenchymal transition in AML-12 cells.
MedLine Citation:
PMID:  19779649     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cortactin is an F-actin binding protein, regulating cell movement and adhesive junction assembly. However, the function of cortactin in epithelial-mesenchymal transition (EMT) remains elusive. Here we found that during transforming growth factor-beta1 (TGF-beta1)- induced EMT in AML-12 murine hepatocytes, cortactin underwent tyrosine dephosphorylation. Inhibition of the dephosphorylation of cortactin by sodium vanadate blocked TGF-beta1-induced EMT. Knockdown of cortactin by RNAi led to decrease of intercellular junction proteins E-cadherin and Zonula occludens-1 and induced expression of mesenchymal protein fibronectin. Additionally, knockdown of cortactin further promoted TGF-beta1-induced EMT in AML-12 cells, as determined by EMT markers and cell morphological changes. Moreover, migration assay showed that cortactin knockdown promoted the migration of AML-12 cells, and also enhanced TGF-beta1-induced migration. Our study showed the involvement of cortactin in the TGFbeta1- induced EMT.
Authors:
Kehua Zhang; Dongmei Wang; Jianguo Song
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta biochimica et biophysica Sinica     Volume:  41     ISSN:  1745-7270     ISO Abbreviation:  Acta Biochim. Biophys. Sin. (Shanghai)     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-09-25     Completed Date:  2010-02-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101206716     Medline TA:  Acta Biochim Biophys Sin (Shanghai)     Country:  China    
Other Details:
Languages:  eng     Pagination:  839-45     Citation Subset:  IM    
Affiliation:
Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
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MeSH Terms
Descriptor/Qualifier:
Cell Differentiation / physiology*
Cell Transdifferentiation / physiology*
Cortactin / physiology*
Epithelial Cells / physiology
Histone Acetyltransferases / physiology
Humans
Kidney / cytology*
Mesoderm / physiology
Nerve Tissue Proteins / physiology
Transforming Growth Factor beta1 / physiology*
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Cortactin; 0/Nerve Tissue Proteins; 0/Transforming Growth Factor beta1; EC 2.3.1.48/ELP3 protein, human; EC 2.3.1.48/Histone Acetyltransferases

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