Document Detail


Correlative nanoscale imaging of actin filaments and their complexes.
MedLine Citation:
PMID:  23727693     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Actin remodeling is an area of interest in biology in which correlative microscopy can bring a new way to analyze protein complexes at the nanoscale. Advances in EM, X-ray diffraction, fluorescence, and single molecule techniques have provided a wealth of information about the modulation of the F-actin structure and its regulation by actin binding proteins (ABPs). Yet, there are technological limitations of these approaches to achieving quantitative molecular level information on the structural and biophysical changes resulting from ABPs interaction with F-actin. Fundamental questions about the actin structure and dynamics and how these determine the function of ABPs remain unanswered. Specifically, how local and long-range structural and conformational changes result in ABPs induced remodeling of F-actin needs to be addressed at the single filament level. Advanced, sensitive and accurate experimental tools for detailed understanding of ABP-actin interactions are much needed. This article discusses the current understanding of nanoscale structural and mechanical modulation of F-actin by ABPs at the single filament level using several correlative microscopic techniques, focusing mainly on results obtained by Atomic Force Microscopy (AFM) analysis of ABP-actin complexes.
Authors:
Shivani Sharma; Huanqi Zhu; Elena E Grintsevich; Emil Reisler; James K Gimzewski
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Review     Date:  2013-06-03
Journal Detail:
Title:  Nanoscale     Volume:  5     ISSN:  2040-3372     ISO Abbreviation:  Nanoscale     Publication Date:  2013 Jul 
Date Detail:
Created Date:  2013-06-17     Completed Date:  2014-01-17     Revised Date:  2014-05-27    
Medline Journal Info:
Nlm Unique ID:  101525249     Medline TA:  Nanoscale     Country:  England    
Other Details:
Languages:  eng     Pagination:  5692-702     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Actin Cytoskeleton / chemistry*,  ultrastructure*
Animals
Humans
Microfilament Proteins / chemistry*
Microscopy, Atomic Force / methods*
Multiprotein Complexes / chemistry*,  ultrastructure*
Portraits as Topic
Grant Support
ID/Acronym/Agency:
R01 GM 077190/GM/NIGMS NIH HHS; R01 GM077190/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Microfilament Proteins; 0/Multiprotein Complexes
Comments/Corrections

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