Document Detail

Correlative light-electron microscopy as a tool to study in vivo dynamics and ultrastructure of intracellular structures.
MedLine Citation:
PMID:  23027014     Owner:  NLM     Status:  In-Data-Review    
Correlative light-electron microscopy (CLEM) is a very effective technique that combines live-cell imaging and immuno-electron microscopy for ultrastructural morphological characterization of dynamic intracellular organelles. The use of green fluorescent protein (GFP)-tagged chimeras allows the user to follow the movements and/or behavior of intracellular structures in a live cell and to fix it at the moment of interest. The subsequent immuno-electron microscopy processing can then reveal the three-dimensional architecture of the same structure, together with precise recognition of the GFP-labeled protein. The process resembles the taking of a high-resolution snapshot of an interesting live scene. Considering that CLEM is a very useful but technically demanding and time-consuming technique, accurate protocols will be helpful to simplify the work of scientists who are willing to apply this method for their own purposes. Here, we present a detailed protocol that describes all of the "tricks" and know-hows involved in carrying out the crucial steps of a CLEM experiment.
Elena V Polishchuk; Roman S Polishchuk; Alberto Luini
Related Documents :
23059554 - Energy transfer cassettes based on organic fluorophores: construction and applications ...
24460244 - Multifunctional two-photon active silica-coated au@mno janus particles for selective du...
24373954 - An ultrasensitive homogeneous aptasensor for kanamycin based on upconversion fluorescen...
16514594 - Allostery of the two-state model of hemoglobin studied by ecepp energy minimization.
20482204 - Differential conductance and quantum interference in kondo systems.
21693904 - Summary of workshop 'theory meets industry'-the impact of ab initio solid state calcul...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  931     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-10-02     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  413-22     Citation Subset:  IM    
Institute of Protein Biochemistry, Naples, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  A novel combined imaging/morphometrical method for the analysis of human sural nerve biopsies for cl...
Next Document:  Photooxidation technology for correlative light and electron microscopy.