| Correlations Between FAS Elongation Cycle Genes Expression and Fatty Acid Production for Improvement of Long-Chain Fatty Acids in Escherichia coli. | |
| | |
MedLine Citation:
|
PMID: 23322253 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Microorganisms have been used for biodiesel (fatty acid methyl ester) production due to their significant environmental and economic benefits. The aim of the present research was to develop new strains of Escherichia coli K-12 MG1655 and to increase the content of long-chain fatty acids by overexpressing essential enzymes that are involved in the fatty acid synthase elongation cycle. In addition, the relationship of β-ketoacyl-acyl carrier protein (ACP) synthase (fabH), β-ketoacyl-ACP reductase (fabG), β-hydroxyacyl-ACP dehydrase (fabZ), and β-enoyl-ACP reductase (fabI) with respect to fatty acid production was investigated. The four enzymes play a unique role in fatty acid biosynthesis and elongation processes. We report the generation of recombinant E. coli strains that produced long-chain fatty acids to amounts twofold over wild type. To verify the results, NAD(+)/NADH ratios and glucose analyses were performed. We also confirmed that FabZ plays an important role in producing unsaturated fatty acids (UFAs) as E. coli SGJS25 (overexpressing the fabZ gene) produced the highest percentage of UFAs (35 % of total long-chain fatty acids), over wild type and other recombinants. Indeed, cis-9-hexadecenoic acid, a major UFA in E. coli SGJS25, was produced at levels 20-fold higher than in wild type after 20 h in culture. The biochemically engineered E. coli presented in this study is expected to be more economical for producing long-chain fatty acids in quality biodiesel production processes. |
| | |
Authors:
|
Sunhee Lee; Yeontae Jung; Seunghan Lee; Jinwon Lee |
Related Documents
:
|
1517723 - Are papanicolaou smears enough? acetic acid washes of the cervix as adjunctive therapy:... 12969493 - Fermentation of cellulose to acetic acid by clostridium lentocellum sg6: induction of s... 18287353 - In vitro micellarization and intestinal cell uptake of cis isomers of lycopene exceed t... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2013-1-17 |
Journal Detail:
|
Title: Applied biochemistry and biotechnology Volume: - ISSN: 1559-0291 ISO Abbreviation: Appl. Biochem. Biotechnol. Publication Date: 2013 Jan |
Date Detail:
|
Created Date: 2013-1-16 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8208561 Medline TA: Appl Biochem Biotechnol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Department of Chemical and Biomolecular Engineering, Sogang University, Seoul, 121-742, Republic of Korea. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Bovine Serum Albumin Nanospheres Synchronously Encapsulating "Gold Selenium/Gold" Nanoparticles and ...
Next Document: Ethanol Production Using Whole Plant Biomass of Jerusalem Artichoke by Kluyveromyces marxianus CBS15...