Document Detail

Correlation of p-glycoprotein detection by immunohistochemistry with mdr-1 mRNA levels in osteosarcomas. Pilot study.
MedLine Citation:
PMID:  7735558     Owner:  NLM     Status:  MEDLINE    
All the factors that influence prognosis in patients with osteosarcomas have not been fully determined. One reported predictor of poor outcome is increased multi-drug resistant gene (mdr-1) expression, as measured by reverse transcription and polymerase chain reaction (RT-PCR). We examined whether immunostaining for p-glycoprotein, the protein product of mdr-1, could be used instead of RT-PCR to indicate the presence of the multidrug-resistant phenotype. The sensitivity of the immunostaining was determined using KB cell sublines. For 13 cases of osteosarcoma, samples were immunostained for p-glycoprotein and the levels of mdr-1 expression quantitated with use of RT-PCR. Three osteosarcomas with undetectable levels of mdr-1 expression by RT-PCR were negative immunohistochemically. Ten cases showed mdr-1 expression ranging from approximately 1 to 32 copies of mdr-1 mRNA/cell. Of these cases, five cases contained occasional tumour cells with positive immunostaining. There was no correlation between levels of expression and the presence or number of immunoreactive cells. These results indicate that the presence of p-glycoprotein immunostaining does not reliably correlate with the level of mdr-1 expression. However it may be useful in conjunction with RT-PCR to further define different subgroups of osteosarcomas that may have different prognoses, and this is currently under investigation.
R A Kandel; S Campbell; S Noble-Topham; R Bell; I L Andrulis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Diagnostic molecular pathology : the American journal of surgical pathology, part B     Volume:  4     ISSN:  1052-9551     ISO Abbreviation:  Diagn. Mol. Pathol.     Publication Date:  1995 Mar 
Date Detail:
Created Date:  1995-06-07     Completed Date:  1995-06-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9204924     Medline TA:  Diagn Mol Pathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  59-65     Citation Subset:  IM    
Department of Pathology, Mount Sinai Hospital, University of Toronto, Ontario, Canada.
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MeSH Terms
Base Sequence
Bone Neoplasms / chemistry,  genetics
Cell Line
Immunoenzyme Techniques
KB Cells
Molecular Sequence Data
Osteosarcoma / chemistry*,  genetics
P-Glycoprotein / analysis*,  genetics
RNA, Messenger / analysis*
RNA, Neoplasm / analysis
Reg. No./Substance:
0/P-Glycoprotein; 0/RNA, Messenger; 0/RNA, Neoplasm

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