Document Detail


Correlation of metabolic stability and altered quaternary structure of oncoprotein p53 with cell transformation.
MedLine Citation:
PMID:  1984409     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The phosphoprotein p53 seems to be implicated in various processes connected with cell transformation and in particular with the regulation of cell cycle and probably DNA replication. In the present paper we have analyzed two sets of closely related cell lines expressing the same p53 which exhibited either a nontransformed or a transformed phenotype. These cell lines were used to study biochemical properties of the p53 protein which might be correlated with cell transformation. We found a positive correlation among an elevated stability of p53, the formation of high-molecular-weight forms of p53, and the transformed phenotype of the corresponding cell lines. Furthermore, these data indicate that self-aggregation prevents p53 from rapid degradation. By a comparative analysis of the stability and oligomerization properties of mutant p53 and wild-type p53, we could demonstrate that elevated stability and self-aggregation of p53 are correlated with the transformed phenotype of the cells and independent of a particular mutation in the p53 gene.
Authors:
S Kraiss; S Spiess; E Reihsaus; M Montenarh
Related Documents :
20664969 - Oridonin induces g2/m cell cycle arrest and apoptosis through mapk and p53 signaling pa...
17457979 - Kn-93, a specific inhibitor of camkii inhibits human hepatic stellate cell proliferatio...
19934289 - Interactions of the hdm2/p53 and proteasome pathways may enhance the antitumor activity...
12826669 - Hyperproliferation and p53 status of lens epithelial cells derived from alphab-crystall...
8979269 - Clonal variation of p53 expression and proliferative phenotype in a253 squamous carcino...
15001399 - Differential regulation of signal transduction pathways in wild type and mutated p53 br...
25485709 - Metallothionein blocks oxidative dna damage induced by acute inorganic arsenic exposure.
9154519 - Synchronous bursting can arise from mutual excitation, even when individual cells are n...
10409289 - Metallothionein response in gills of oreochromis mossambicus exposed to copper in fresh...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental cell research     Volume:  192     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1991 Jan 
Date Detail:
Created Date:  1991-01-30     Completed Date:  1991-01-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  157-64     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, University of Ulm, Federal Republic of Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Cell Line, Transformed
Cell Transformation, Neoplastic / metabolism*
Macromolecular Substances
Phenotype
Tumor Suppressor Protein p53 / chemistry*,  metabolism
Chemical
Reg. No./Substance:
0/Macromolecular Substances; 0/Tumor Suppressor Protein p53

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Highly synchronous culture of fibroblasts from G2 block caused by staurosporine, a potent inhibitor ...
Next Document:  Inhibition of cell proliferation by the cholecystokinin antagonist L-364,718.