Document Detail


Correlation of membrane lipid peroxidation with oxidation of hemoglobin variants: possibly related to the rates of hemin release.
MedLine Citation:
PMID:  8791096     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Experiments were performed to delineate the biochemical mechanism of hemoglobin (Hb)-catalyzed lipid peroxidation in human red blood cells (RBCs). Using a modified Langmuir trough lipid monolayer technique, we found that oxidized Hb induced an increase in lipid monolayer surface pressure, suggesting that oxidized Hb readily releases its heme moiety into the lipid monolayer. To confirm our interpretation that oxidized Hb readily releases its heme moiety, we monitored the fluorescence of Hb tryptophan upon oxidation of Hb. We found an increase in Hb fluorescence in the aqueous phase of our monolayer system after the addition of H2O2. The increase in fluorescence should reflect the departure of heme from globin due to a decrease in fluorescent quenching effect by the heme moiety. The rate of increase in lipid monolayer surface pressure upon Hb oxidation differed from Hb to Hb with an order of Hb E > F > S > A. The ability of various Hbs to affect lipid peroxidation in the RBC membrane, as monitored by the parinaric acid oxidation technique, followed this same order. In addition, hemin was shown to be a more potent catalyst of lipid peroxidation in RBC membrane than nonheme irons.
Authors:
D T Chiu; J van den Berg; F A Kuypers; I J Hung; J S Wei; T Z Liu
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Free radical biology & medicine     Volume:  21     ISSN:  0891-5849     ISO Abbreviation:  Free Radic. Biol. Med.     Publication Date:  1996  
Date Detail:
Created Date:  1997-02-12     Completed Date:  1997-02-12     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8709159     Medline TA:  Free Radic Biol Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  89-95     Citation Subset:  IM    
Affiliation:
Chang Gung College of Medicine and Technology, Tao-yuan, Taiwan, ROC.
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MeSH Terms
Descriptor/Qualifier:
Adult
Animals
Brain
Cattle
Erythrocyte Membrane / metabolism
Erythrocytes / metabolism*
Fetal Hemoglobin / metabolism
Genetic Variation
Hemin / metabolism*
Hemoglobin A / metabolism
Hemoglobin E / metabolism
Hemoglobin, Sickle / metabolism
Hemoglobins / drug effects,  metabolism*
Humans
Hydrogen Peroxide / pharmacology
Kinetics
Lipid Peroxidation*
Liposomes
Oxyhemoglobins / drug effects,  metabolism*
Phospholipids
Pressure
Spectrometry, Fluorescence
Surface Properties
Thiobarbituric Acid Reactive Substances / analysis
Grant Support
ID/Acronym/Agency:
HL 20985/HL/NHLBI NIH HHS; HL 36255/HL/NHLBI NIH HHS; HL 55213/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Hemoglobin, Sickle; 0/Hemoglobins; 0/Liposomes; 0/Oxyhemoglobins; 0/Phospholipids; 0/Thiobarbituric Acid Reactive Substances; 16009-13-5/Hemin; 7722-84-1/Hydrogen Peroxide; 9034-51-9/Hemoglobin A; 9034-61-1/Hemoglobin E; 9034-63-3/Fetal Hemoglobin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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