| Correlation of membrane glucocorticoid receptor levels with glucocorticoid-induced apoptotic competence using mutant leukemic and lymphoma cells lines. | |
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MedLine Citation:
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PMID: 12244567 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have studied the presence and functional implications of membrane glucocorticoid receptor (mGR) in several wild type (WT) and mutant mouse lymphoid cell lines (nuclear transfer decrease, NT(-); nuclear transfer increase, NT(i); and receptorless, R(-)). Direct fluorescent antibody staining revealed large aggregates of mGR-specific fluorescing antigens in the plasma membrane of the WT and mGR-enriched (mGR(++)) S-49 cells. While R(-) cells totally lacked mGR, this receptor level was low in NT(-) and NT(i) groups. FACS analysis corroborated these results, showing a approximately 4-10-fold difference between the highest mGR levels (mGR(++)) and the R(-) and NT(i) cells. Membrane extracts were analyzed for mGR by immunoblotting. Multiple receptor forms, ranging in M(r) from 94,000 to > 200,000, were observed in the WT cells, while only smaller peptides (85,000-94,000) were found in NT(-) cells. No detectable immunoreactive bands were identified in either membrane or cytosol immunoprecipitates of NT(i) and R(-) cell groups. Within 48 h post dexamethasone exposure > 98% of WT and mGR(++) S-49 cells underwent apoptosis, compared to 0-30% in the mutant cells, albeit the total receptor number is two to three times higher in NT(i) compared to WT. These results suggest a better correlation between the quantity and quality of mGRs (rather than total cellular GRs) and the ability of glucocorticoids (GCs) to lyse lymphoid cells. |
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Authors:
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Bahiru Gametchu; Cheryl S Watson |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of cellular biochemistry Volume: 87 ISSN: 0730-2312 ISO Abbreviation: J. Cell. Biochem. Publication Date: 2002 |
Date Detail:
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Created Date: 2002-09-23 Completed Date: 2003-03-14 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8205768 Medline TA: J Cell Biochem Country: United States |
Other Details:
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Languages: eng Pagination: 133-46 Citation Subset: IM |
Copyright Information:
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Copyright 2002 Wiley-Liss, Inc. |
Affiliation:
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Department of Pediatrics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA. gametchu@mcw.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / drug effects*, physiology Binding Sites Blotting, Western Cell Membrane / metabolism Centrifugation, Density Gradient / methods Dexamethasone / pharmacology Flow Cytometry Fluorescent Antibody Technique, Direct Glucocorticoids / pharmacology* Humans Immunoblotting Leukemia / metabolism*, pathology Lymphoma / metabolism*, pathology Mice Protein Binding Receptors, Glucocorticoid / genetics, metabolism*, physiology Statistics as Topic Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
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65674//PHS HHS |
| Chemical | |
Reg. No./Substance:
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0/Glucocorticoids; 0/Receptors, Glucocorticoid; 50-02-2/Dexamethasone |
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