Document Detail


Correlation of locomotor activity and brain infarction in rats with transient focal ischemia.
MedLine Citation:
PMID:  19917312     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Occlusion of middle cerebral artery (MCAo) is commonly used to generate stroke in experimental animals. Different behavioral assays have been used to evaluate the severity of lesions or recovery after treatment in this model. In this study, we examined the correlation between the size of infarction and locomotor activity after transient MCAo in adult Sprague-Dawley rats. The right middle cerebral artery was occluded for 30-90-min by ligation with a 10-O suture. At 2 days after MCAo, animals were individually placed in a 3-D infrared activity chamber for 1h to measure their horizontal, vertical, stereotypic and rotational activity. Body asymmetry was examined using an elevated body swing test. Volume of infarction was measured by triphenyltetrazolium chloride staining. We found that there is a significant correlation between the volume of infarction and vertical movement, such as vertical activity, vertical movement number and vertical movement time. There is also a significant correlation between the infarction volume and body asymmetry. In conclusion, our data suggest that vertical locomotor activity and body asymmetry are useful behavioral indices to estimate the size of lesioning in rodents after MCAo.
Authors:
Hui Shen; Yun Wang
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural     Date:  2009-11-13
Journal Detail:
Title:  Journal of neuroscience methods     Volume:  186     ISSN:  1872-678X     ISO Abbreviation:  J. Neurosci. Methods     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-01     Completed Date:  2010-04-20     Revised Date:  2012-03-08    
Medline Journal Info:
Nlm Unique ID:  7905558     Medline TA:  J Neurosci Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  150-4     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Elsevier B.V. All rights reserved.
Affiliation:
National Institute on Drug Abuse, IPR, Baltimore, MD 21224, USA. hshen@intra.nida.nih.gov
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain / pathology,  physiopathology*
Brain Infarction / pathology,  physiopathology
Disease Models, Animal
Functional Laterality
Infarction, Middle Cerebral Artery / pathology,  physiopathology*
Infrared Rays
Ischemic Attack, Transient / pathology,  physiopathology*
Male
Motor Activity / physiology*
Rats
Stereotyped Behavior / physiology
Tetrazolium Salts
Time Factors
Grant Support
ID/Acronym/Agency:
Z01 DA000429-09/DA/NIDA NIH HHS; Z01 DA000429-10/DA/NIDA NIH HHS; Z01 DA000461-05/DA/NIDA NIH HHS; Z01 DA000461-06/DA/NIDA NIH HHS; Z99 DA999999/DA/NIDA NIH HHS
Chemical
Reg. No./Substance:
0/Tetrazolium Salts; 902-00-1/triphenyltetrazolium
Comments/Corrections

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