Document Detail


Correlation of invasion and metastasis of cancer cells, and expression of the RAD21 gene in oral squamous cell carcinoma.
MedLine Citation:
PMID:  16416296     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although RAD21 is involved in the repair of double-strand breaks in DNA and is essential for mitotic growth, its role in cancer has been unclear. In this study, the relevance of RAD21 gene expression to the invasion and metastasis of oral squamous cell carcinoma was clarified using laser microdissection and real-time polymerase chain reaction (PCR). Using two different metastatic potential oral squamous cells [high-metastatic-potential squamous cell carcinoma cells (SAS-Ly) and low-metastatic-potential squamous cell carcinoma cells (SAS)], the relation of RAD21 gene expression to apoptosis, invasion, and metastasis was examined. The results showed that RAD21 gene expression was significantly decreased in oral squamous cell carcinoma when it expressed the INFbeta and INFgamma invasion patterns in comparison with the INFalpha invasion pattern (p<0.01). In addition, in comparison with SAS cells, SAS-Ly cells indicated tolerance to cell death induced by an apoptosis induction reagent, while the expression level of the RAD21 gene in SAS cells was increased by the apoptosis induction reagent. However, in SAS-Ly cells, the reagent induced no significant difference. Our findings indicate that the RAD21 gene was closely related to the invasion and metastasis of cancer cells.
Authors:
Gou Yamamoto; Tarou Irie; Tadateru Aida; Yuuki Nagoshi; Reiko Tsuchiya; Tetsuhiko Tachikawa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-01-14
Journal Detail:
Title:  Virchows Archiv : an international journal of pathology     Volume:  448     ISSN:  0945-6317     ISO Abbreviation:  Virchows Arch.     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-04-04     Completed Date:  2007-08-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9423843     Medline TA:  Virchows Arch     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  435-41     Citation Subset:  IM    
Affiliation:
Department of Oral Pathology and Diagnosis, School of Dentistry, Showa University, Shinagawaku, Tokyo, Japan. gd0021@dent.showa-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Aged, 80 and over
Animals
Apoptosis / drug effects
Carcinoma, Squamous Cell / drug therapy,  genetics,  pathology*
Cell Line, Tumor
Cell Survival / drug effects
Dactinomycin / pharmacology
Female
Gene Expression Regulation, Neoplastic*
Humans
Hydroxamic Acids / pharmacology
Male
Mice
Mice, Nude
Middle Aged
Mouth Neoplasms / drug therapy,  genetics,  pathology*
Neoplasm Invasiveness
Nuclear Proteins / genetics*,  metabolism
Phosphoproteins / genetics*,  metabolism
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Chemical
Reg. No./Substance:
0/Hydroxamic Acids; 0/Nuclear Proteins; 0/Phosphoproteins; 0/RAD21 protein, human; 0/RNA, Messenger; 50-76-0/Dactinomycin; 58880-19-6/trichostatin A

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