Document Detail


Correlation in the human aorta of APO B fractions with tissue cholesterol and collagen content.
MedLine Citation:
PMID:  223586     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The amounts of buffer- and Triton-extracted apo B (LDL-protein), as well as the sum of these two fractions, were correlated with the total tissue cholesterol and hydroxyproline content (as a measure of collagen) in grossly normal intima, fatty streaks, and fibrous plaques of human aortas obtained at autopsy. Quantitative values of buffer- and Triton-extracted apo B were obtained by sequentially extracting homogenates of aortic intima with an aqueous buffer and one containing Triton X-100, and measuring the apo B content in each extract by an electroimmunoassay relative to plasma LDL or Triton-treated LDL. Significant positive correlations were obtained between the following: tissue cholesterol and both buffer-extracted and total-extracted apo B in grossly normal intima; tissue cholesterol and Triton-extracted apo B in microdissected fibrotic caps and cores of fibrous plaques, as well as in whole plaques. A positive correlation was also obtained between tissue cholesterol and total-extracted apo B in the necrotic core. A significant negative correlation was found between Triton-extracted apo B and collagen in whole plaques. The calculated mean percent of total tissue cholesterol in the different aortic regions that could be present as part of an intact LDL particle were: 100% in grossly normal intima, 16% in fatty streaks, and 11% in fibrous plaques. The positive correlation between Triton-extracted apo B and cholesterol in plaques suggests one or both of the following: the extracellular pool of cholesterol or some material increasing concurrently with cholesterol interacts with apo B or another part of the LDL particle; or the apo B containing lipoprotein is trapped in the hydrophobic environment of extracellular lipid. Both possibilities would render the particle less soluble in aqueous buffers. The negative correlation between Triton-extracted apo B and tissue collagen and the lack of a significant correlation between buffer-extracted apo B and collagen content suggests that collagen is probably not responsible for apo B retention in the aortic intima.
Authors:
H F Hoff; M Karagas; C L Heideman; J W Gaubatz; A M Gotto
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Atherosclerosis     Volume:  32     ISSN:  0021-9150     ISO Abbreviation:  Atherosclerosis     Publication Date:  1979 Mar 
Date Detail:
Created Date:  1979-09-17     Completed Date:  1979-09-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0242543     Medline TA:  Atherosclerosis     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  259-68     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Aorta / metabolism*
Apolipoproteins / metabolism*
Arteriosclerosis / metabolism
Cholesterol / metabolism*
Collagen / metabolism*
Humans
Lipoproteins, LDL / metabolism
Chemical
Reg. No./Substance:
0/Apolipoproteins; 0/Lipoproteins, LDL; 57-88-5/Cholesterol; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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