Document Detail


Correlation of immunohistochemical p53 labeling index with inhibition rate in chemosensitivity test in gastric and colon cancer.
MedLine Citation:
PMID:  11299739     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To determine whether the expression of p53, p21, bcl-2 or Ki-67 in cancer cells is predictive of chemosensitivity, immunohistochemical examination of these factors and chemosensitivity assays were performed on colon and gastric cancer specimens. Chemosensitivity tests were performed using CDDP, 5-FU, MMC, or ADR and inhibition rate (IR) was calculated by MTT assay. Before exposure to anticancer drugs, the samples were investigated immunohistochemically for expression of the above factors and after anticancer drug exposure by TUNNEL staining, for the presence of apoptotic cells. With 5-FU and MMC, the apoptotic index was well correlated with IR, so their effects were related to apoptosis. Moreover, with these two agents, the p53 labeling index (LI) was inversely correlated with IR and p21-LI showed a good correlation with IR. We therefore concluded that immunohistochemical studies for p53 and p21 were useful for predicting the chemosensitivities of colon and gastric cancer to MMC and 5-FU.
Authors:
N Hosaka; Y Ichikawa; T Ishikawa; Y Nagashima; C Kunisaki; M Takahashi; Y Moriwaki; H Akiyama; S Yamaguchi; M Ota; S Ooki; H Ike; H Shimada
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Anticancer research     Volume:  21     ISSN:  0250-7005     ISO Abbreviation:  Anticancer Res.     Publication Date:    2001 Jan-Feb
Date Detail:
Created Date:  2001-04-12     Completed Date:  2001-05-03     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  229-35     Citation Subset:  IM    
Affiliation:
Second Department of Surgery and Department of Pathology, Yokohama City University, School of Medicine, 3-9 Fukuura Kanazawa-ku, Yokohama, Japan.
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents / pharmacology*
Apoptosis / drug effects
Cisplatin / pharmacology
Colonic Neoplasms / drug therapy*,  metabolism,  pathology
Cyclin-Dependent Kinase Inhibitor p21
Cyclins / metabolism
Doxorubicin / pharmacology
Drug Screening Assays, Antitumor
Fluorouracil / pharmacology
Forecasting
Humans
Immunohistochemistry
Ki-67 Antigen / metabolism
Mitomycin / pharmacology
Proto-Oncogene Proteins c-bcl-2 / metabolism
Stomach Neoplasms / drug therapy*,  metabolism,  pathology
Tumor Suppressor Protein p53 / metabolism*
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/CDKN1A protein, human; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Cyclins; 0/Ki-67 Antigen; 0/Proto-Oncogene Proteins c-bcl-2; 0/Tumor Suppressor Protein p53; 15663-27-1/Cisplatin; 23214-92-8/Doxorubicin; 50-07-7/Mitomycin; 51-21-8/Fluorouracil

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