| Correlation of immunohistochemical p53 labeling index with inhibition rate in chemosensitivity test in gastric and colon cancer. | |
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MedLine Citation:
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PMID: 11299739 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To determine whether the expression of p53, p21, bcl-2 or Ki-67 in cancer cells is predictive of chemosensitivity, immunohistochemical examination of these factors and chemosensitivity assays were performed on colon and gastric cancer specimens. Chemosensitivity tests were performed using CDDP, 5-FU, MMC, or ADR and inhibition rate (IR) was calculated by MTT assay. Before exposure to anticancer drugs, the samples were investigated immunohistochemically for expression of the above factors and after anticancer drug exposure by TUNNEL staining, for the presence of apoptotic cells. With 5-FU and MMC, the apoptotic index was well correlated with IR, so their effects were related to apoptosis. Moreover, with these two agents, the p53 labeling index (LI) was inversely correlated with IR and p21-LI showed a good correlation with IR. We therefore concluded that immunohistochemical studies for p53 and p21 were useful for predicting the chemosensitivities of colon and gastric cancer to MMC and 5-FU. |
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Authors:
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N Hosaka; Y Ichikawa; T Ishikawa; Y Nagashima; C Kunisaki; M Takahashi; Y Moriwaki; H Akiyama; S Yamaguchi; M Ota; S Ooki; H Ike; H Shimada |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Anticancer research Volume: 21 ISSN: 0250-7005 ISO Abbreviation: Anticancer Res. Publication Date: 2001 Jan-Feb |
Date Detail:
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Created Date: 2001-04-12 Completed Date: 2001-05-03 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 8102988 Medline TA: Anticancer Res Country: Greece |
Other Details:
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Languages: eng Pagination: 229-35 Citation Subset: IM |
Affiliation:
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Second Department of Surgery and Department of Pathology, Yokohama City University, School of Medicine, 3-9 Fukuura Kanazawa-ku, Yokohama, Japan. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Antineoplastic Agents
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pharmacology* Apoptosis / drug effects Cisplatin / pharmacology Colonic Neoplasms / drug therapy*, metabolism, pathology Cyclin-Dependent Kinase Inhibitor p21 Cyclins / metabolism Doxorubicin / pharmacology Drug Screening Assays, Antitumor Fluorouracil / pharmacology Forecasting Humans Immunohistochemistry Ki-67 Antigen / metabolism Mitomycin / pharmacology Proto-Oncogene Proteins c-bcl-2 / metabolism Stomach Neoplasms / drug therapy*, metabolism, pathology Tumor Suppressor Protein p53 / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents; 0/CDKN1A protein, human; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Cyclins; 0/Ki-67 Antigen; 0/Proto-Oncogene Proteins c-bcl-2; 0/Tumor Suppressor Protein p53; 15663-27-1/Cisplatin; 23214-92-8/Doxorubicin; 50-07-7/Mitomycin; 51-21-8/Fluorouracil |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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