Document Detail


Correlation of house dust mite-specific lymphocyte proliferation with IL-5 production, eosinophilia, and the severity of symptoms in infants with atopic dermatitis.
MedLine Citation:
PMID:  9449505     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Because house dust mite (HDM)-specific IgE antibody (IgE-RAST) is usually not detectable in infants with atopic dermatitis (AD), HDM has not been regarded as the cause of infantile AD. The level of HDM-specific lymphocyte proliferation (expressed as stimulation index measured by flow cytometry [SIF]), however, was found to be markedly elevated in AD infants. This suggests that the sensitization of T cells to HDM extract occurs even in infancy. Moreover, the level of HDM-SIF is correlated closely with the severity of infantile AD, suggesting that HDM is a major cause of this disease not only in adults and children but also in infants. Although the level of HDM-SIF did not correlate with the level of HDM-specific IgE-RAST in infants with AD, it did intimately correlate with the absolute number of peripheral blood eosinophils. Because T lymphocytes are known to secrete some cytokines, such as IL-5, that enhance the proliferation of eosinophils, we measured the IL-5 production by peripheral blood mononuclear cells in infants with AD on stimulation with HDM extract. The amount of IL-5 production is significantly higher in infants with AD, as well as in children with AD, than that found in nonatopic control subjects. Moreover, the level of IL-5 production is correlated closely with the level of HDM-SIF in infants with AD. Taken together, these results suggest that HDMs play an important role in the development of infantile AD by inducing IL-5 production from HDM-specific T lymphocytes.
Authors:
M Kimura; S Tsuruta; T Yoshida
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  The Journal of allergy and clinical immunology     Volume:  101     ISSN:  0091-6749     ISO Abbreviation:  J. Allergy Clin. Immunol.     Publication Date:  1998 Jan 
Date Detail:
Created Date:  1998-02-05     Completed Date:  1998-02-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1275002     Medline TA:  J Allergy Clin Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  84-9     Citation Subset:  AIM; IM    
Affiliation:
Department of Allergy and Clinical Immunology, Shizuoka Children's Hospital, Urushiyama, Shizuoka City, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Age Factors
Allergens / adverse effects
Animals
Candida albicans / immunology
Child
Child, Preschool
Dermatitis, Atopic / etiology*,  immunology
Dust / adverse effects*
Eosinophilia / etiology*,  immunology
Humans
Immunoglobulin E / blood
Infant
Interleukin-5 / biosynthesis*
Lymphocyte Activation*
Mites / immunology*
Chemical
Reg. No./Substance:
0/Allergens; 0/Dust; 0/Interleukin-5; 37341-29-0/Immunoglobulin E

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