Document Detail

Correlation of gastric mucosal damage with sialic acid profile in rats: effect of hydrochloric acid, pepsin and hypertonic saline.
MedLine Citation:
PMID:  15515124     Owner:  NLM     Status:  MEDLINE    
Sialic acids occupy terminal positions on gastric mucus glycoprotein where they contribute to the high viscosity of mucin. Desialylation of mucus may lead to degradation of the mucus and eventually to the breakdown of the gastric mucus barrier. The effect of a variety of damaging agents (0.1 M HCl, 2 mg ml(-1) pepsin and 2 M NaCl) on sialic acid profile was determined in pylorus-ligated rats. The relationship between sialic acid, galactose, pyruvate and the extent of gastric mucosal damage were studied. Instillation of pepsin significantly increased total sialic acid, galactose and macroscopic mucosal lesions in the stomach. Instillation of 0.1 M HCl reduced the total sialic acid but this decrease was not significant. Acidity led to a significant increase in the amount of free sialic acid in the gastric instillates and the macroscopic lesions induced by acid was not significantly different from the control animals (0.15 M NaCl). 2 M NaCl induced the macroscopic lesions in the stomach and also free sialic acid in the instillates. Pepsin potentiates the action of 2 M NaCl. In all the agents examined with the exception of acid, it was observed that an increase in free sialic acid and galactose was accompanied by gastric mucosal erosion and elevation of pyruvate concentration. It is concluded that gastric acidity alone is not inherently damaging and that resistance of gastric mucosa to destructive agents may be dependent on the integrity of the sialic acids.
Sadiq Yusuf; Andrew J Nok; Danladi A Ameh; Alexander B Adelaiye; Emmanuel O Balogun
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cell biochemistry and function     Volume:  23     ISSN:  0263-6484     ISO Abbreviation:  Cell Biochem. Funct.     Publication Date:    2005 Sep-Oct
Date Detail:
Created Date:  2005-08-30     Completed Date:  2005-12-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8305874     Medline TA:  Cell Biochem Funct     Country:  England    
Other Details:
Languages:  eng     Pagination:  339-45     Citation Subset:  IM    
Department of Human Physiology, Faculty of Medicine, Ahmadu Bello University, Zaria, Kaduna State, Nigeria.
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MeSH Terms
Galactose / metabolism
Gastric Mucosa / drug effects*,  metabolism,  pathology*
Hydrochloric Acid / pharmacology*
N-Acetylneuraminic Acid / analysis,  metabolism*
Pepsin A / pharmacology*
Pyruvic Acid / metabolism
Rats, Wistar
Saline Solution, Hypertonic / pharmacology*
Reg. No./Substance:
0/Saline Solution, Hypertonic; 127-17-3/Pyruvic Acid; 131-48-6/N-Acetylneuraminic Acid; 26566-61-0/Galactose; 7647-01-0/Hydrochloric Acid; EC A

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