Document Detail

Correlation of biochemical blocks and genetic lesions in leucine auxotrophic strains of the imperfect yeast Candida maltosa.
MedLine Citation:
PMID:  1865875     Owner:  NLM     Status:  MEDLINE    
The four enzymatic steps in the conversion of alpha-ketoisovaleriate to leucine were examined in the wild type and in 13 leucine auxotrophic strains of Candida maltosa. The genetic lesions in the auxotrophs, involve at least five different loci and are correlated with three enzymatic steps. This was confirmed by gene cloning, protoplast fusion, and enzyme assays. The pathway for leucine biosynthesis in C. maltosa shows general similarity to that of other lower eukaryotes but there are individual differences in the numbers of genes responsible for single enzymatic steps. A disomic state of the chromosomes carrying genes coding for alpha-isopropylmalate synthase and beta-isopropyl-malate dehydrogenase was elucidated.
D Becher; H Wedler; H Schulze; R Bode; A Kasüske; I Samsonova
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular & general genetics : MGG     Volume:  227     ISSN:  0026-8925     ISO Abbreviation:  Mol. Gen. Genet.     Publication Date:  1991 Jul 
Date Detail:
Created Date:  1991-09-12     Completed Date:  1991-09-12     Revised Date:  2006-05-01    
Medline Journal Info:
Nlm Unique ID:  0125036     Medline TA:  Mol Gen Genet     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  361-8     Citation Subset:  IM    
Fachrichtung Biologie, Ernst-Moritz-Arndt-Universität Greifswald, FRG.
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MeSH Terms
2-Isopropylmalate Synthase / genetics
Candida / genetics*
Cloning, Molecular
DNA Probes
DNA, Fungal / genetics
Electrophoresis, Agar Gel
Genes, Fungal*
Leucine / metabolism*
Restriction Mapping
Reg. No./Substance:
0/DNA Probes; 0/DNA, Fungal; 61-90-5/Leucine; EC Synthase

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