| Correlation between tissue growth kinetics and modulation of mouse skin tumorigenesis by phorbol esters. | |
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MedLine Citation:
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PMID: 1816077 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Previous studies on the influence of phorbol esters on mouse skin tumorigenesis have shown that 12-O-tetradecanoylphorbol-13-acetate (TPA) enhances development of malignant epithelial and mesenchymal skin tumors by a completely carcinogenic dose of 3-methylcholanthrene (MCA), while its congener phorbol-12, 13-diacetate (PDA) exerts an inhibitory effect. Differential effects of these two agents were analysed by histology, morphometry and cell kinetic techniques including autoradiography and estimation of labelled precursor incorporation into DNA by liquid scintillation counting. Epidermal hyperplasia induced on exposure of S/RV Cri mouse skin to a single or multiple TPA application after MCA injection was associated with a significant increase in the thickness of nucleated cell layers, stratum granulosum, number of suprabasal cells and dark basal cells. Enhancing effect of TPA on MCA-induced neoplastic development correlated well with an increase in mitotic activity, number of cells in S-phase and increased rate of DNA synthesis in the epidermis, dermis and subcutis as also mast cell number. In contrast, treatment of MCA-injected preneoplastic mouse skin with PDA resulted in epidermal hypoplasia and cellular damage evident as cytoplasmic vacuolation and nuclear pyknosis. Multiple PDA exposure also reduced the thickness, mitotic index and number of cells in S-phase in epidermis, dermis and subcutis. Thus, cellular toxicity and inability to recruit cells in DNA-synthetic phase may account for inhibition of progression of preneoplastic epithelial and mesenchymal cells into overt tumors by PDA. |
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Authors:
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A G Ramchandani; R A Bhisey |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Indian journal of experimental biology Volume: 29 ISSN: 0019-5189 ISO Abbreviation: Indian J. Exp. Biol. Publication Date: 1991 Nov |
Date Detail:
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Created Date: 1992-06-26 Completed Date: 1992-06-26 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0233411 Medline TA: Indian J Exp Biol Country: INDIA |
Other Details:
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Languages: eng Pagination: 1010-6 Citation Subset: IM |
Affiliation:
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Carcinogenesis Division, Tata Memorial Centre, Bombay, India. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Division Female Kinetics Mice Mice, Inbred Strains Papilloma / chemically induced* Phorbol Esters / pharmacology* Skin Neoplasms / chemically induced*, pathology |
| Chemical | |
Reg. No./Substance:
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0/Phorbol Esters |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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