| Correlation between silver-stained nucleolar organizer region area and cell cycle time. | |
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MedLine Citation:
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PMID: 11169575 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: The relationship between the population doubling time and the quantity of silver-stained nucleolar organizer region (AgNOR) interphase proteins was studied in cell culture at three different temperatures used to modulate the cell cycle duration. METHODS: After MIB 1 and AgNOR combined staining, the quantity of AgNOR proteins was measured in cycling cells by image cytometry. RESULTS: Among the several parameters calculated, the AgNOR relative area showed a strong correlation with the changes of the population doubling time induced by different temperatures. CONCLUSIONS: The results support the hypothesis that the cell cycle time and the size of the ribogenesis machinery are coregulated and that measurements of AgNORs can thus be used as a static evaluation of the cell cycle duration in arbitrary units. |
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Authors:
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V Canet; M P Montmasson; Y Usson; F Giroud; G Brugal |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Cytometry Volume: 43 ISSN: 0196-4763 ISO Abbreviation: Cytometry Publication Date: 2001 Feb |
Date Detail:
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Created Date: 2001-02-22 Completed Date: 2001-04-19 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 8102328 Medline TA: Cytometry Country: United States |
Other Details:
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Languages: eng Pagination: 110-6 Citation Subset: IM |
Copyright Information:
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Copyright 2001 Wiley-Liss, Inc. |
Affiliation:
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Laboratoire TIMC-IMAG, Institut Albert Bonniot, Université Joseph Fourier Grenoble I, La Tronche, France. Veronique.Canet@imag.fr |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, Nuclear Autoantigens / analysis Biological Markers / analysis Cell Cycle / genetics* Cell Division / genetics Humans Image Cytometry / statistics & numerical data Ki-67 Antigen Nuclear Proteins / analysis Nucleolus Organizer Region / chemistry*, genetics, metabolism Silver Staining / methods*, statistics & numerical data Temperature Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Nuclear; 0/Autoantigens; 0/Biological Markers; 0/Ki-67 Antigen; 0/Nuclear Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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