| Correlation between oestrogen receptor protein expression in infiltrating ductal carcinoma of the breast by immunohistochemistry and cytosol measurements. | |
| | |
MedLine Citation:
|
PMID: 9522973 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Fresh frozen neoplastic tissues from 70 infiltrating ductal breast carcinomas were analysed for cytosolic oestrogen receptor (ER) protein content using a solid phase enzyme immunoassay (EIA) method based on a "sandwich" principle (Abbott ER-EIA monoclonal). Formalin-fixed, paraffin-embedded sections from the same carcinomas were examined for nuclear immunoreactivity against a monoclonal antibody for ER protein (Dako) using the standard avidin-biotin complex immunoperoxidase (IP) method after microwave antigen retrieval. The degree of ER positivity by IP was also scored according to a visual estimation of the percentage of cells expressing immunopositivity and the intensity of staining. Twenty-eight (40%) of the carcinomas were ER-positive by EIA and 34 (48.6%) were positive by IP. Twenty-five (35.7%) were ER-positive and 33 (47.1%) were ER-negative by both methods. Nine (12.9%) were ER-negative by EIA but were positive by IP, this discrepancy being ascribed to sampling inadequacy for EIA. However, 3 (4.3%) tumours were ER-positive by EIA and negative by IP. This discrepancy may be variously due to inadequate antigen retrieval, faulty technique and the possibility that the two methods do not measure identical ER proteins. IP appears to have an advantage over EIA in that it has a higher pick-up rate, does not require fresh tissue and can be applied to archival material. However, to reduce false negative estimations, it may be necessary to run IP staining using more than one ER antibody. Standardisation of the IP method for ER is desirable before this method is to be widely adopted in Malaysian laboratories. Quantitation of ER positivity by IP scoring correlated poorly with actual cytosolic levels. Caution should be exercised in attaching patient management value to visual IP scoring. |
| | |
Authors:
|
L M Looi; S F Yap; P L Cheah |
Related Documents
:
|
8338483 - A comparison between radioligand and immunohistochemical assay of hormone receptors in ... 12579263 - Expression of a novel splicing variant deleting exons 4 and 6 of the progesterone recep... 15318923 - Low-molecular-weight cyclin e: the missing link between biology and clinical outcome. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Annals of the Academy of Medicine, Singapore Volume: 26 ISSN: 0304-4602 ISO Abbreviation: Ann. Acad. Med. Singap. Publication Date: 1997 Nov |
Date Detail:
|
Created Date: 1998-05-07 Completed Date: 1998-05-07 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 7503289 Medline TA: Ann Acad Med Singapore Country: SINGAPORE |
Other Details:
|
Languages: eng Pagination: 750-3 Citation Subset: IM |
Affiliation:
|
Department of Pathology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Breast Neoplasms
/
chemistry* Carcinoma, Ductal, Breast / chemistry* Female Humans Immunoenzyme Techniques Receptors, Cytoplasmic and Nuclear / analysis* Receptors, Estrogen / analysis* |
| Chemical | |
Reg. No./Substance:
|
0/Receptors, Cytoplasmic and Nuclear; 0/Receptors, Estrogen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Ultrasound of the tibia--precision error, left versus right sides and correlation with bone mineral ...
Next Document: The subcellular localisation and the time course of bismuth in the gastric mucosa of rats after shor...