Document Detail


Correlation between the molecular weight and potency of polar compounds which induce the differentiation of HL-60 human promyelocytic leukemia cells.
MedLine Citation:
PMID:  3466688     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Structure-activity studies of a series of polar organic compounds, including N,N-dimethylformamide, N-methylformamide, and related ureas and acetamides, were performed with regard to their ability to promote the terminal differentiation of the human promyelocytic leukemia cell line HL-60 to granulocyte-like cells. Functional and morphological criteria were used to assess the percentage of differentiated cells which arose from their continuous incubation with different concentrations of each agent over a period of 96 h. All of the alkylformamides, alkylacetamides, and alkylureas tested were found to induce differentiation, regardless of structure. Inspection of the results showed that there was a linear relationship (r = -0.937) between the molecular weight of each compound and the logarithm of the concentration which was required to bring about the differentiation of the greatest number of cells, while viability was generally maintained at greater than 85%. Once established, this relationship was used to predict the potency of several polar solvents which were structurally unrelated to the formamides. For example, methanol, ethanol, and acetone were all inducers of differentiation with a potency predictable from their molecular weight alone. The terminal differentiation induced by all of the compounds was only accomplished by cells which were capable of replication prior to differentiation. At concentrations which prevented a single replication and brought about a fall in cell viability over 96 h, no differentiation was observed. A correlation was observed between the molecular weight of each compound and the logarithm of its concentration to bring about cytotoxicity without differentiation (r = -0.935), and the line was almost parallel to that defining the concentration required for optimal differentiation (slope values of -0.02126 and -0.02288). A poorer (r = -0.6654) correlation was found between the logarithm of the octanol-water partition coefficient and the logarithm of the concentration required for optimal differentiation, when the data for 12 of the polar organic compounds were analyzed. The results suggest that no special structural requirements are necessary for the alkylformamides, -acetamides, -ureas, and related compounds to induce the terminal differentiation of HL-60 cells to granulocyte-like cells, but that the activity of each compound could be predicted from their molecular weight. The concentrations required to induce differentiation were marginally lower than those which were cytostatic or cytotoxic, which suggested that a toxic threat to the cells was sufficient to induce differentiation.(ABSTRACT TRUNCATED AT 400 WORDS)
Authors:
S P Langdon; J A Hickman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer research     Volume:  47     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1987 Jan 
Date Detail:
Created Date:  1987-02-02     Completed Date:  1987-02-02     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  140-4     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Differentiation / drug effects
Cell Line
Dose-Response Relationship, Drug
Humans
Leukemia, Myeloid, Acute / pathology*
Molecular Weight
Solubility
Solvents / pharmacology*
Structure-Activity Relationship
Chemical
Reg. No./Substance:
0/Solvents

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