Document Detail

Correlation between lifetime heterogeneity and kinetics heterogeneity during chlorophyll fluorescence induction in leaves: 2. Multi-frequency phase and modulation analysis evidences a loosely connected PSII pigment-protein complex.
MedLine Citation:
PMID:  15238211     Owner:  NLM     Status:  MEDLINE    
We report the first direct decomposition of the fluorescence lifetime heterogeneity during multiphasic fluorescence induction in dark-adapted leaves by multi-frequency phase and modulation fluorometry (PMF). A very fast component, assigned to photosystem I (PSI), was found to be constant in lifetime and yield, whereas the two slow components, which are strongly affected by the closure of the reaction centers by light, were assigned to PSII. Based on a modified "reversible radical pair" kinetic model with three compartments, we showed that a loosely connected pigment complex, which is assumed to be the CP47 complex, plays a specific role with respect to the structure and function of the PSII: (i) it explains the heterogeneity of PSII fluorescence lifetime as a compartmentation of excitation energy in the antenna, (ii) it is the site of a conformational change in the first second of illumination, and (iii) it is involved in the mechanisms of nonphotochemical quenching (NPQ). On the basis of the multi-frequency PMF analysis, we reconciled two apparently antagonistic aspects of chlorophyll a fluorescence in vivo: it is heterogeneous with respect to the kinetic structure (several lifetime components) and homogeneous with respect to average quantities (quasi-linear mean tau-Phi relationship).
Nicolae Moise; Ismaël Moya
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1657     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2004 Jun 
Date Detail:
Created Date:  2004-07-07     Completed Date:  2004-08-17     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  47-60     Citation Subset:  IM    
Laboratoire pour l'Utilisation du Rayonnement Electromagnétique, Centre Universitaire Paris-Sud, Bat. 209D, B.P. 34, 91898 Orsay Cedex, France.
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MeSH Terms
Adaptation, Physiological / physiology,  radiation effects
Chlorophyll / metabolism*
Photosystem II Protein Complex / metabolism*,  radiation effects*
Plant Leaves / metabolism*,  radiation effects*
Proteins / metabolism,  radiation effects
Prunus / metabolism,  radiation effects
Reproducibility of Results
Sensitivity and Specificity
Spectrometry, Fluorescence / instrumentation,  methods*
Statistics as Topic
Reg. No./Substance:
0/Photosystem II Protein Complex; 0/Proteins; 1406-65-1/Chlorophyll; 479-61-8/chlorophyll a

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