Document Detail

Correlation between lifetime heterogeneity and kinetics heterogeneity during chlorophyll fluorescence induction in leaves: 1. Mono-frequency phase and modulation analysis reveals a conformational change of a PSII pigment complex during the IP thermal phase.
MedLine Citation:
PMID:  15238210     Owner:  NLM     Status:  MEDLINE    
The relationship between the fluorescence lifetime (tau) and yield (Phi) obtained in phase and modulation fluorometry at 54 MHz during the chlorophyll fluorescence induction in dark-adapted leaves under low actinic light has been investigated. Three typical phases have been identified: (i) linear during the OI photochemical rise, (ii) convex curvature during the subsequent IP thermal rise, and (iii) linear during the PS slow decay. A similar relationship has been obtained in the fluorescence induction for the fluorescence yield measured at 685 nm plotted versus the fluorescence yield measured at 735 nm. A spectrally resolved analysis shows that the curvature of the tau-Phi relationship is not due to chlorophyll fluorescence reabsorption effects. Several other hypotheses are discussed and we conclude that the curvature of the tau-Phi relationship is due to a variable and transitory nonphotochemical quenching. We tentatively propose that this quenching results from a conformational change of a pigment-protein complex of Photosystem II core antenna during the IP phase and could explain both spectral and temporal transitory changes of the fluorescence. A variable blue shift of the 685 nm peak of the fluorescence spectrum during the IP phase has been observed, supporting this hypothesis.
Nicolae Moise; Ismaël Moya
Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't; Validation Studies    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1657     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2004 Jun 
Date Detail:
Created Date:  2004-07-07     Completed Date:  2004-08-17     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  33-46     Citation Subset:  IM    
Laboratoire pour l'Utilisation du Rayonnement Electromagnétique, Centre Universitaire Paris-Sud, Bat. 209D, 91898 Orsay, France.
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MeSH Terms
Adaptation, Physiological / physiology,  radiation effects
Chlorophyll / metabolism*
Hordeum / metabolism,  radiation effects
Peas / metabolism,  radiation effects
Plant Leaves / metabolism*,  radiation effects*
Prunus / metabolism,  radiation effects
Reproducibility of Results
Sensitivity and Specificity
Spectrometry, Fluorescence / instrumentation,  methods*
Statistics as Topic
Tobacco / metabolism,  radiation effects
Reg. No./Substance:
1406-65-1/Chlorophyll; 479-61-8/chlorophyll a

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