Document Detail


Correlation between hepatitis B viremia and the clinical and histological activity of chronic delta hepatitis.
MedLine Citation:
PMID:  7997189     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To analyze the serological, clinical and histological significance of hepatitis B virus (HBV) replication among a group of patients with chronic delta hepatitis (CDH), we have studied the clinical and the histological activity in 49 patients with CDH. The HBV-DNA was analyzed by dot-blot and polymerase chain reaction (PCR). Concomitant infection with hepatitis C virus (HCV) was analyzed by reverse transcriptase (RT)-PCR, HDV replication by dot-blot, and human immunodeficiency virus (HIV) infection by enzyme-linked immunosorbent assay. The subjects were divided into three groups according to HBV-DNA status: group I: 14 patients HBV-DNA dot-blot positive; group II: 29 patients HBV-DNA positive only by PCR, and group III: 6 patients HBV-DNA negative by dot-blot and PCR. We have found HBV-DNA by dot-blot in 28.5% of patients, and by PCR in 87.7%. Also 22 patients were anti-HCV positive (86.3% had HCV-RNA by RT-PCR). The first group (HBV-DNA dot-blot positive) had significantly higher serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) than those in the second and third groups. Likewise, serum ALT and AST were significantly higher in the second group (HBV-DNA positive by PCR) than in those of the third group. Histological inflammatory activity was significantly higher in the group of patients with HBV-DNA detectable by dot-blot. The prevalence of serum HDV-RNA and IgM anti-HDV were similar in the three groups. These results were similar in the anti-HCV-positive and -negative patients. In conclusion, these data suggest that: (1) persistence of HBV replication is a major determinant of severe liver damage in chronic delta hepatitis, and (2) HCV and HIV infections do not influence the natural history of CDH.
Authors:
J L Lozano; J Crespo; F de la Cruz; F Casafont; M J Lopez-Arias; L Martín-Ramos; F Pons-Romero
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Medical microbiology and immunology     Volume:  183     ISSN:  0300-8584     ISO Abbreviation:  Med. Microbiol. Immunol.     Publication Date:  1994 Jul 
Date Detail:
Created Date:  1995-01-17     Completed Date:  1995-01-17     Revised Date:  2007-02-21    
Medline Journal Info:
Nlm Unique ID:  0314524     Medline TA:  Med Microbiol Immunol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  159-67     Citation Subset:  IM; X    
Affiliation:
Gastroenterology and Hepatology Unit, University Hospital Marqués de Valdecilla, Faculty of Medicine, Santander, Spain.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Base Sequence
Chronic Disease
DNA Probes
DNA, Viral / analysis
Female
HIV Infections / pathology
Hepacivirus / genetics,  immunology,  physiology
Hepatitis B / pathology*
Hepatitis B virus / genetics,  immunology,  physiology
Hepatitis C / pathology
Hepatitis D / pathology*
Hepatitis Delta Virus / genetics,  immunology,  physiology
Humans
Immunoblotting
Immunoglobulin G / analysis
Immunoglobulin M / analysis
Male
Middle Aged
Molecular Sequence Data
Polymerase Chain Reaction
RNA, Viral / analysis
Viremia / pathology*
Virus Replication / physiology
Chemical
Reg. No./Substance:
0/DNA Probes; 0/DNA, Viral; 0/Immunoglobulin G; 0/Immunoglobulin M; 0/RNA, Viral

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