Document Detail


Correlation between growth inhibition and presence of 5'-methylthioadenosine in cells treated with interferon.
MedLine Citation:
PMID:  6426777     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Treatment with interferon of several lines of human and murine cells resulted in a modest increase of the S- adenyosylmethionine level and in a greater increase of the S-adenosylhomocysteine level, with a consequent decrease in the S- adenyosylmethionine /S-adenosylhomocysteine ratio. These results confirmed a previous report (F. de Ferra and C. Baglioni , J. Biol. Chem., 258: 2118-2121, 1983) of such an effect of interferon on HeLa cells. The increase in S- adenyosylmethionine and S-adenosylhomocysteine was not observed in lymphoblastoid Raji cells, which are not growth inhibited by interferon. When Daudi cells and two other cell lines sensitive to growth inhibition by interferon were labeled with [35S]methionine, two novel labeled compounds were detected by high-pressure liquid chromatography analysis of cell extracts. These compounds were not present in untreated cells, and were either absent or present in reduced amount in extracts of cells resistant to the antiproliferative effect of interferon. One of the labeled compounds was tentatively identified with 5'-methylthioadenosine (MTA), a naturally occurring nucleoside, which was previously shown to inhibit murine lymphoid cell proliferation. Addition of MTA to cultures of different cell lines resulted in inhibition of cell growth. When the MTA concentration was measured in Daudi cells treated in this way, a relatively low concentration of MTA was detected, which was comparable to that found in interferon-treated cells. It seems possible that the presence of MTA in interferon-treated cells contributes to the inhibition of cell growth.
Authors:
F de Ferra; C Baglioni
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cancer research     Volume:  44     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1984 Jun 
Date Detail:
Created Date:  1984-07-12     Completed Date:  1984-07-12     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2297-301     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenosine / analogs & derivatives*,  metabolism
Adenosylhomocysteinase
Burkitt Lymphoma
Cell Division / drug effects
Cell Line
Deoxyadenosines*
Hela Cells / drug effects,  physiology
Humans
Hydrolases / metabolism
Interferon Type I / pharmacology*
Kinetics
Purine-Nucleoside Phosphorylase / metabolism
S-Adenosylhomocysteine / metabolism
S-Adenosylmethionine / metabolism
Thionucleosides / metabolism*
Grant Support
ID/Acronym/Agency:
CA-29895/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Deoxyadenosines; 0/Interferon Type I; 0/Thionucleosides; 2457-80-9/5'-methylthioadenosine; 29908-03-0/S-Adenosylmethionine; 58-61-7/Adenosine; 979-92-0/S-Adenosylhomocysteine; EC 2.4.2.1/Purine-Nucleoside Phosphorylase; EC 2.4.2.28/5'-methylthioadenosine phosphorylase; EC 3.-/Hydrolases; EC 3.3.1.1/Adenosylhomocysteinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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