Document Detail

Correlation between erythrocytes deformability and size: a study using a microchannel based cell analyzer.
MedLine Citation:
PMID:  17123552     Owner:  NLM     Status:  MEDLINE    
The deformability of erythrocytes is of great importance for oxygen delivery in the microcirculation [Lipowsky, H.H., 2005. Microvascular rheology and hemodynamics. Microcirculation 12, 5-15]. Aging of erythrocytes is associated with a reduction in deformability and also in size. The present work describes an automated cell analyzer which utilizes a glass microchannel and advanced image processing software. Erythrocytes suspended in a high viscosity medium are filmed flowing through the microchannel. Under these conditions, the cells assume different orientations and undergo varying deformations according to their location in the velocity profile. The cell analyzer enables the measurement of individual erythrocyte velocity, deformability and volume at varying depths within the microchannel. The volume of the cells is calculated based on the experimental data and a fluid mechanics model. The results obtained show that, on average, the deformability of the cells increases with increase in their size. Additionally, the behavior of RBCs in a microchannel is investigated, showing promising diagnostic possibilities.
Avishay Bransky; Natanel Korin; Yael Nemirovski; Uri Dinnar
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-11-22
Journal Detail:
Title:  Microvascular research     Volume:  73     ISSN:  0026-2862     ISO Abbreviation:  Microvasc. Res.     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2007-01-15     Completed Date:  2007-04-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0165035     Medline TA:  Microvasc Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7-13     Citation Subset:  IM    
Biomedical Department and Electrical Engineering Department, Technion, Haifa, Israel.
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MeSH Terms
Cell Size*
Erythrocyte Deformability / physiology*
Erythrocytes / cytology*
Hemorheology / instrumentation,  methods
Image Processing, Computer-Assisted
Microfluidic Analytical Techniques / instrumentation,  methods

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