Document Detail

Correlation between differentiation and lung colonization by retinoic acid-treated F9 cells as revealed by the expression pattern of extracellular matrix and cell surface antigens.
MedLine Citation:
PMID:  3348356     Owner:  NLM     Status:  MEDLINE    
For study of the correlation between differentiation and organ colonization properties of tumor cells, F9 embryonal carcinoma (EC) cells were treated with retinoic acid, an inducer of differentiation; and their organ colonization pattern was assessed by the experimental metastasis assay. Untreated cells were found to colonize the liver, whereas treated cells colonized the lungs. This pattern held true when metastases were scored after spontaneous death or after a careful microscopic search for micrometastases. Histologic examination revealed that both the tumor nodules produced by the untreated and the treated cells had the characteristics of EC devoid of any evidence of differentiation. The immunohistochemical study of the expression of markers typical of embryonal carcinoma cells or of the extracellular matrix components laminin and collagen type IV, typical of differentiated cells, confirmed these results. However, the lack of expression of stage-specific embryonal antigen 1 (SSEA-1), a marker generally associated with the undifferentiated state, observed only in the tumors obtained after injection of treated cells, indicates that the lung nodules probably derive from cells that have responded to the induction in vitro but have dedifferentiated in vivo.
L Leoncini; L Pacenti; D Rusciano; D Burroni; S Garbisa; M Cintorino; B Terrana
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of pathology     Volume:  130     ISSN:  0002-9440     ISO Abbreviation:  Am. J. Pathol.     Publication Date:  1988 Mar 
Date Detail:
Created Date:  1988-04-20     Completed Date:  1988-04-20     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0370502     Medline TA:  Am J Pathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  505-14     Citation Subset:  AIM; IM    
Laboratorio Biologia Cellulare, Centro Ricerche, Sclavo SpA, Siena, Italy.
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MeSH Terms
Antigens, Surface / analysis*
Cell Differentiation / drug effects
Cell Line / drug effects
Collagen / analysis
Extracellular Matrix / analysis*
Laminin / analysis
Liver Neoplasms / pathology
Lung Neoplasms / pathology
Neoplasm Metastasis
Skin Neoplasms / pathology
Teratoma / pathology*
Tretinoin / pharmacology*
Reg. No./Substance:
0/Antigens, Surface; 0/Laminin; 302-79-4/Tretinoin; 9007-34-5/Collagen
Erratum In:
Am J Pathol 1988 Jul;132(1):12

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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