Document Detail


Correlation between cytokine levels of amniotic fluid and histological chorioamnionitis in preterm delivery.
MedLine Citation:
PMID:  9120746     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of this study was to investigate the correlation between the cytokine levels in the amniotic fluid (AF) and the histological stage of chorioamnionitis (CAM) in premature labor. AF of 6 cases (7 samples of AF were obtained as one was a twin pregnancy) in whom CAM was diagnosed histologically, and 12 cases without CAM were included in this study. Amniotic fluid was obtained within 24 hours prior to delivery. Cytokine levels (IL-2, -4, -6, TNF-alpha, IFN-gamma) in AF were measured by an ELISA method. Levels of IL-2 and -6 in the CAM-positive group (mean +/-S.E., 52.9 +/- 83.9 pg/ml, and 20,537.9 +/- 8853.7 pg/ml, respectively) were higher than those in the CAM-negative group (i.e. undetectable, and 65.6 +/- 27.5, respectively) with a statistical significance of p < 0.05, p < 0.001, respectively. There was a positive linear relationship between IL-6 levels of AF and the placental histological inflammatory stages of Blanc in the CAM-positive group. From these results it would appear that the IL-6 level in AF is the most sensitive test in the detection of extraamniotic infection or intraamniotic infection in preterm labor with intact membranes and also indicates the severity infection.
Authors:
H Negishi; H Yamada; M Mikuni; T Kishida; K Okuyama; T Sagawa; S Makinoda; S Fujimoto
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of perinatal medicine     Volume:  24     ISSN:  0300-5577     ISO Abbreviation:  J Perinat Med     Publication Date:  1996  
Date Detail:
Created Date:  1997-04-24     Completed Date:  1997-04-24     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0361031     Medline TA:  J Perinat Med     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  633-9     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynecology, Hokkaido University, School of Medicine, Sapporo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Amniocentesis
Amniotic Fluid / metabolism*
Birth Weight
C-Reactive Protein / metabolism
Chorioamnionitis / diagnosis,  pathology*
Cytokines / metabolism*
Female
Fever
Humans
Infant, Newborn
Interferon-gamma / metabolism
Interleukin-2 / metabolism
Interleukin-4 / metabolism
Interleukin-6 / metabolism
Leukocyte Count
Obstetric Labor, Premature*
Placenta / pathology
Pregnancy
Tumor Necrosis Factor-alpha / metabolism
Chemical
Reg. No./Substance:
0/Cytokines; 0/Interleukin-2; 0/Interleukin-6; 0/Tumor Necrosis Factor-alpha; 207137-56-2/Interleukin-4; 82115-62-6/Interferon-gamma; 9007-41-4/C-Reactive Protein

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