| The Correlation between Composition of the Bacterial Community and Concentration of Volatile Fatty Acids in the Rumen during the Transition Period and Ketosis in Dairy Cows. | |
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MedLine Citation:
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PMID: 22267666 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The transition period is a severe challenge to dairy cows. Glucose supply cannot meet demand and body fat is mobilized, potentially leading to negative energy balance (NEB), ketosis or fatty liver. Propionate produces glucose by gluconeogenesis, which generation depends heavily on the number and species of microbes. In present study we analyzed the rumen microbiome composition of cows in the transition period, cows with ketosis and non-perinatal cows using terminal restriction fragment length polymorphism (TRFLP) analysis of 16S rRNA genes and Quantitative PCR. TRFLP analysis indicated that the quantity of Veillonellaceae was reduced, and that of Streptococcaceae was increased in rumen samples from the transition period and ketosis groups, and Lactobacillaceae was increased after calving. Quantitative PCR data suggested that numbers of the main propionate-producing microbes, Megasphaera elsdenii and Selenomonas ruminantium, were decreased, while numbers of the main lactate-producing bacteria, Streptococcus bovis were increased in the rumen of cows from the transition period and ketosis groups, and Lactobacillus spp. was increased after calving. VFAs and glucose concentrations were decreased, but lactic acid increased, in rumen samples from the transition period and ketosis groups. Our results indicate that the VFAs concentration was significantly related to the numbers of Selenomonus ruminantium and Megasphaera elsdenii in the rumen. |
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Authors:
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Xiaoxu Wang; Xiaobing Li; Chenxu Zhao; Pan Hu; Hui Chen; Zhaoxi Liu; Guowen Liu; Zhe Wang |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-1-20 |
Journal Detail:
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Title: Applied and environmental microbiology Volume: - ISSN: 1098-5336 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-1-23 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi' an Road, Changchun, Jilin, 130062, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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