| Correlation of anisotropic cell behaviors with topographic aspect ratio. | |
| | |
MedLine Citation:
|
PMID: 19118891 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In this study, we have used nanoimprinting to create a range of micro- and nanoscale gratings, or their combination, in bulk polystyrene plates to investigate anisotropic cell behaviors of human dermal fibroblasts with respect to the aspect ratio (depth/width) of gratings. The depth and width of the polystyrene gratings both show strong effects individually on cell alignment and elongation that are qualitatively similar to the results of other studies. However, consistent quantitative comparison of these individual parameters with different studies is complicated by the diversity of combinations of width and depth that have been tested. Instead, the aspect ratio of the gratings as a unified description of grating topography is a more consistent parameter to interpret topographic dependence of cell morphology. Both cell alignment and elongation increase with increasing aspect ratio, and even a shallow grating (aspect ratio of approximately 0.05) is sufficient to induce 80% cell alignment. Re-plotting data recently published by other groups vs. aspect ratio shows a similar dependence, despite differences in cell types and surface structures. This consistency indicates that aspect ratio is a general factor to characterize cell behaviors. The relationship of cell elongation and alignment with topographic aspect ratio is interpreted in terms of the theory of contact guidance. This model provides simplicity and flexibility in geometry design for devices and materials that interface with cells. |
| | |
Authors:
|
Adam S Crouch; D Miller; Kevin J Luebke; W Hu |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-12-31 |
Journal Detail:
|
Title: Biomaterials Volume: 30 ISSN: 1878-5905 ISO Abbreviation: Biomaterials Publication Date: 2009 Mar |
Date Detail:
|
Created Date: 2009-01-23 Completed Date: 2009-03-27 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: England |
Other Details:
|
Languages: eng Pagination: 1560-7 Citation Subset: IM |
Affiliation:
|
Department of Electrical Engineering, University of Texas at Dallas, Richardson, TX 75080, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Anisotropy Collagen / pharmacology Fibroblasts / cytology*, drug effects Humans Male Microscopy, Atomic Force Microscopy, Electron, Scanning Microscopy, Fluorescence Polystyrenes |
| Chemical | |
Reg. No./Substance:
|
0/Polystyrenes; 9007-34-5/Collagen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A sensitive and efficient detection method for bovine viral diarrhea virus (BVDV) in single preimpla...
Next Document: The role of the silicatein-alpha interactor silintaphin-1 in biomimetic biomineralization.