Document Detail


Correlation of actinomycin X2 to the lipid profile in static and shaken cultures of Streptomyces nasri strain YG62.
MedLine Citation:
PMID:  10581734     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Streptomyces nasri strain YG62 produces a broad-spectrum antibiotic designated actinomycin X2. The influence of static and shaken incubation on the production of actinomycin X2 and lipid profiles of S. nasri strain YG62 was investigated. It was found that shaken incubation was superior to the static process for both actinomycin X2 (2-fold) and total lipids (1.6-fold). Triglyceride and phospholipid levels paralleled the actinomycin X2 production with an increase in the triglyceride (2.8-fold) and phospholipid (1.2-fold) concentrations in the shaken culture over the static incubation. Analysis of fatty acid patterns revealed the occurrence of a wide range of fatty acids (C10-C22). The mean percentage of total saturated fatty acids in shaken culture was higher than those of the static culture. The mean percentage of mono-unsaturated fatty acids was almost the same in both cultures. The mean percentage of the total polyunsaturated fatty acids in the static culture was slightly higher than that of the shaken culture. The polyunsaturated/saturated fatty acid ratio (P/S) was higher in the static culture compared with the shaken culture. A positive correlation was recorded between triglycerides, phospholipids and actinomycin X2. A negative correlation on the other hand, was found between fatty acids and actinomycin X2.
Authors:
M Y el-Naggar; M A el-Kersh; A S el-Sharaky
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Microbios     Volume:  100     ISSN:  0026-2633     ISO Abbreviation:  Microbios     Publication Date:  1999  
Date Detail:
Created Date:  2000-02-04     Completed Date:  2000-02-04     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0207257     Medline TA:  Microbios     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  117-27     Citation Subset:  IM    
Affiliation:
Botany and Microbiology Department, Faculty of Science, Alexandria University, Egypt.
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MeSH Terms
Descriptor/Qualifier:
Anti-Bacterial Agents / biosynthesis*
Chromatography, Gas
Culture Media
Fatty Acids / chemistry,  metabolism
Lipid Metabolism*
Lipids / chemistry
Microbiological Techniques
Oxazines / metabolism*
Peptides, Cyclic / biosynthesis*
Phospholipids / chemistry,  metabolism
Streptomyces / growth & development,  metabolism*
Triglycerides / chemistry,  metabolism
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Culture Media; 0/Fatty Acids; 0/Lipids; 0/Oxazines; 0/Peptides, Cyclic; 0/Phospholipids; 0/Triglycerides

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