| The correlation between intestinal gonadotropin-releasing hormone (GnRH) and proglucagon in hyperlipidemic rats and Goto-Kakizaki (GK) rats. | |
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MedLine Citation:
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PMID: 19669946 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Our experiment investigated the mRNA expression of intestinal gonadotropin-releasing hormone (GnRH), proglucagon (PG), and glucagon-like peptide 1 receptor (GLP-1R) in the jejunum, ileum, and colon of rats fed with high-fat diet and Goto-Kakizaki (GK) rats and revealed the physiological role of intestinal GnRH. We found that the GnRH and PG mRNA levels in high-cholesterol (HCh) diet were higher than in the control. However, the GnRH receptor (GnRHR) and GLP-1R mRNA levels did not differ significantly between HCh and control. The GnRH, PG, and GLP-1R mRNA levels in GK rats were lower, respectively, than those in control rats, while the GnRHR levels did not differ significantly between GK rats and control rats. There were no difference in GnRH, PG, GnRHR, and GLP-1R mRNA levels in the ileum and colon tissue between HCh and control rats. The GnRH mRNA levels of GK rats were lower than those in control rats; however, the PG, GLP-1R, and GnRHR levels did not differ significantly between GK and control rats. The GLP-1R mRNA levels of GK rats were lower than those in control rats. The GnRH mRNA expression showed positive correlation with PG mRNA expression in different intestinal sections. The GnRH level in the jejunum showed a significant effect on blood glucose level, while the PG level in the jejunum showed a significant effect on insulin level. This may imply that, compared with the ileum and colon, the jejunum had greater impact on glucose metabolism; furthermore, GnRH might interact with intestinal GLP-1 and GLP-2 through the paracrine and autocrine ways and then regulate glucose metabolism and insulin secretion. |
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Authors:
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Li Wang; Jing Wu; Hongwei Cao; Rong Chen; Nanyan Zhang; Jianfang Fu; Bin Gao; Jing Zhang; Rongrong Hou; Chaowu Tang; Qiuhe Ji |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Endocrine pathology Volume: 20 ISSN: 1559-0097 ISO Abbreviation: Endocr. Pathol. Publication Date: 2009 |
Date Detail:
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Created Date: 2009-11-06 Completed Date: 2010-03-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9009288 Medline TA: Endocr Pathol Country: United States |
Other Details:
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Languages: eng Pagination: 227-34 Citation Subset: IM |
Affiliation:
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Department of Endocrinology, Xijing Hospital, The Fourth Military Medical University, Xi'an, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Glucose / analysis Cholesterol, Dietary / administration & dosage Colon / chemistry Diabetes Mellitus, Type 2 / metabolism* Glucose Tolerance Test Gonadotropin-Releasing Hormone / genetics* Hyperlipidemias / metabolism* Ileum / chemistry Insulin / blood Intestines / chemistry* Jejunum / chemistry Male Proglucagon / genetics* RNA, Messenger / analysis* Rats Rats, Wistar Receptors, Glucagon / genetics Receptors, LHRH / genetics Reverse Transcriptase Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/Blood Glucose; 0/Cholesterol, Dietary; 0/RNA, Messenger; 0/Receptors, Glucagon; 0/Receptors, LHRH; 0/glucagon-like peptide receptor; 11061-68-0/Insulin; 33515-09-2/Gonadotropin-Releasing Hormone; 55963-74-1/Proglucagon |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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