| Correction of the deviations in the retention times with Chromolith columns associated to the flow rate: Implications in the modelling of the retention behaviour. | |
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MedLine Citation:
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PMID: 21360663 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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In a previous work (J. Sep. Sci. 2009, 32, 2793-2803), we reported an interpretive optimisation approach to achieve maximal resolution in minimal analysis time, based on models describing the retention and peak shape as a function of mobile phase composition and flow rate. The method was applied to the separation of a group of basic drugs in a Chromolith column. In that work, we found that the retention factors were sensitive to the flow rate. The reason of the observed deviations in retention times is the increase in the column volume at the applied pressure, which decreases the linear velocity inside the column. This behaviour forced to include a correction term in the model that described the retention. We show here how the deviations in retention times can be evaluated, allowing retention models that do not include the flow rate as a variable, similar to isocratic chromatography at fixed flow rate. The logarithm of the deviations in the retention times with flow rate is shown to correlate with the solute polarity. This correlation is compared with similar correlations for the retention factor at fixed mobile phase composition and the extrapolated retention factor in water at fixed flow rate. |
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Authors:
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Sandra Pous-Torres; José Ramón Torres-Lapasió; María José Ruiz-Ángel; María Celia García-Álvarez-Coque |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-2-25 |
Journal Detail:
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Title: Journal of separation science Volume: - ISSN: 1615-9314 ISO Abbreviation: - Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-3-1 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101088554 Medline TA: J Sep Sci Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Affiliation:
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Departament de Química Analítica, Universitat de València, Burjassot, Spain. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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