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Coronary effluent from postconditioned hearts promotes survival of mesenchymal stem cells under hypoxia.
MedLine Citation:
PMID:  24472011     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Abstract Objectives. Mesenchymal stem cells are sensitive to hypoxia under myocardial micro-environment of ischemia and reperfusion. Ischemic postconditioning, which is cardioprotective against ischemia-reperfusion injury, enhances in vivo survival and therapeutic effects of transplanted stem cells. In this study, we investigated the effects of coronary effluent from postconditioned rat hearts on proliferation and survival of mesenchymal stem cells in vitro under hypoxia. Design. Isolated perfused rat hearts were divided into 3 groups (n=6): the Sham group: receiving a 90 min perfusion; the Control group: receiving a 30 min global ischemia followed by a 60 min reperfusion; the ischemic postconditioning group: before sustained reperfusion, 3 cycles of 30 sec reperfusion and 30 sec ischemia were performed. Inflammation-related factors in coronary effluent were assessed by ELISA. Mesenchymal stem cells from bone marrow of Sprague-Dawley rats were cultured with coronary effluent under hypoxia (95% nitrogen, 5% carbon dioxide, and <1% oxygen) for 6 or 18 hours. Cell proliferation was determined by methyl thiazolyl tetrazolium. Survival rate was measured by Annexin V/PI. Results: Compared with ischemia-reperfusion treatment alone, postconditioning treatment increased the level of interleukin-10 and decreased the level of tumor necrosis factor-α, interleukin-1β in coronary effluent (P<0.01). Stem cells cultured with postconditioned effluent, compared with those with ischemia-reperfusion effluent, had a higher proliferation (optical density value), more surviving cells and less necrosis (P<0.01). Conclusions. Coronary effluent from postconditioned hearts may promote the proliferation and survival of mesenchymal stem cells under hypoxia, and the suppression of inflammation may be involved in this process.
Authors:
Jun Fang; Lin Fan; Lianglong Chen; Xiangqi Chen; Lingzhen Wu
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-1-28
Journal Detail:
Title:  Scandinavian cardiovascular journal : SCJ     Volume:  -     ISSN:  1651-2006     ISO Abbreviation:  Scand. Cardiovasc. J.     Publication Date:  2014 Jan 
Date Detail:
Created Date:  2014-1-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9708377     Medline TA:  Scand Cardiovasc J     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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