| Cornus kousa F.Buerger ex Miquel increases glucose uptake through activation of peroxisome proliferator-activated receptor γ and insulin sensitization. | |
| | |
MedLine Citation:
|
PMID: 21070843 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
AIM OF THE STUDY: Cornus kousa F.Buerger ex Miquel, an oriental medicinal plant, has been traditionally used for the treatment of hyperglycemia, but its molecular mechanism remains unknown. The goal of this study was to investigate the peroxisome proliferator-activated receptor γ (PPARγ) ligand-binding activity of Cornus kousa and to determine the effects of Cornus kousa on insulin sensitization in 3T3-L1 cells for the treatment of type 2 diabetes. MATERIALS AND METHODS: PPARγ luciferase transactivation assay was used to evaluate the PPARγ ligand-binding activity of Cornus kousa leaf extract. Western blot analysis, oil Red O staining, and glucose uptake assay were performed to evaluate PPARγ agonistic activity and insulin sensitizing effects of Cornus kousa leaf extract (CKE) in 3T3-L1 cells. RESULTS: CKE increased PPARγ ligand-binding activity in a dose-dependent manner. In addition, CKE enhanced adipogenesis and the expression of PPARγ target proteins, including glucose transporter 4 (GLUT4) and adiponectin, as well as proteins involved in adipogenesis, including PPARγ and CCAAT/enhancer binding protein α (C/EBPα) in 3T3-L1 adipocytes. Furthermore, CKE led to significant induction of glucose uptake and stimulated insulin signaling, but not to activation of AMP-activated protein kinase (AMPK) signaling. The enhanced glucose uptake by CKE were abolished by treatment with bisphenol a diglycidyl ether (BADGE), a PPARγ antagonist, or LY294002, an inhibitor of phosphoinositide 3-kinase (PI3K), but not by compound C, an AMPK inhibitor. CONCLUSION: Consistent with the high PPARγ ligand-binding activity, CKE increased glucose uptake through PPARγ activation and insulin signaling. These results suggest that CKE could have pharmacological effects for the treatment of hyperglycemia and type 2 diabetes. |
| | |
Authors:
|
Daeyoung Kim; Kwang-Kyun Park; Sang Kook Lee; Seung-Eun Lee; Jae-Kwan Hwang |
Related Documents
:
|
19664593 - Metabolic memory effect of the saturated fatty acid, palmitate, in monocytes. 3527163 - Sphingolipids inhibit insulin and phorbol ester stimulated uptake of 2-deoxyglucose. 2264823 - Acute effects of corticosterone on tissue protein synthesis and insulin-sensitivity in ... |
Publication Detail:
|
Type: Journal Article Date: 2010-11-09 |
Journal Detail:
|
Title: Journal of ethnopharmacology Volume: 133 ISSN: 1872-7573 ISO Abbreviation: J Ethnopharmacol Publication Date: 2011 Jan |
Date Detail:
|
Created Date: 2011-01-17 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7903310 Medline TA: J Ethnopharmacol Country: Ireland |
Other Details:
|
Languages: eng Pagination: 803-9 Citation Subset: IM |
Copyright Information:
|
Copyright © 2010 Elsevier Ireland Ltd. All rights reserved. |
Affiliation:
|
Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, Republic of Korea. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Oral drug delivery in personalized medicine: unmet needs and novel approaches.
Next Document: Anti-inflammatory effects of iridoid glycosides fraction of Folium syringae leaves on TNBS-induced c...