Document Detail


Corneal stroma PDGF blockade and myofibroblast development.
MedLine Citation:
PMID:  19133260     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Myofibroblast development and haze generation in the corneal stroma is mediated by cytokines, including transforming growth factor-beta (TGF-beta), and possibly other cytokines. This study examined the effects of stromal PDGF-beta blockade on the development of myofibroblasts in response to -9.0 diopter photorefractive keratectomy in the rabbit. Rabbits that had haze generating photorefractive keratectomy (PRK, for 9 diopters of myopia) in one eye were divided into three different groups: stromal application of plasmid pCMV.PDGFRB.23KDEL expressing a subunit of PDGF receptor b (domains 2-3, which bind PDGF-B), stromal application of empty plasmid pCMV, or stromal application of balanced salt solution (BSS). The plasmids (at a concentration 1000ng/microl) or BSS was applied to the exposed stroma immediately after surgery and every 24h for 4-5 days until the epithelium healed. The group treated with pCMV.PDGFRB.23KDEL showed lower alphaSMA+ myofibroblast density in the anterior stroma compared to either control group (P<or=0.001). Although there was also lower corneal haze at the slit lamp at one month after surgery, the difference in haze after PDGF-B blockade was not statistically significant compared to either control group. Stromal PDGF-B blockade during the early postoperative period following PRK decreases stromal alphaSMA+ myofibroblast generation. PDGF is an important modulator of myofibroblast development in the cornea.
Authors:
Harmeet Kaur; Shyam S Chaurasia; Fabricio W de Medeiros; Vandana Agrawal; Marcella Q Salomao; Nirbhai Singh; Balamurali K Ambati; Steven E Wilson
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2008-12-24
Journal Detail:
Title:  Experimental eye research     Volume:  88     ISSN:  1096-0007     ISO Abbreviation:  Exp. Eye Res.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-04-27     Completed Date:  2009-10-08     Revised Date:  2014-09-15    
Medline Journal Info:
Nlm Unique ID:  0370707     Medline TA:  Exp Eye Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  960-5     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Actins / metabolism
Animals
Corneal Opacity / etiology,  metabolism*,  pathology
Corneal Stroma / metabolism*,  pathology
Female
Fibroblasts / metabolism,  pathology
Genetic Vectors
Photorefractive Keratectomy / adverse effects
Plasmids
Rabbits
Receptor, Platelet-Derived Growth Factor beta / antagonists & inhibitors*,  genetics,  metabolism
Transfection
Grant Support
ID/Acronym/Agency:
EY015638/EY/NEI NIH HHS; EY10056/EY/NEI NIH HHS; R01 EY010056/EY/NEI NIH HHS; R01 EY010056-14/EY/NEI NIH HHS; R24 EY015638/EY/NEI NIH HHS; R24 EY015638-05/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
0/Actins; EC 2.7.10.1/Receptor, Platelet-Derived Growth Factor beta
Comments/Corrections

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