| Corneal epithelial wound healing in partial limbal deficiency. | |
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MedLine Citation:
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PMID: 1694836 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Previous studies have shown that the corneal epithelial stem cells are located at the limbal basal layer. The limbal stem cells are regarded as the ultimate source for corneal epithelial cell proliferation and differentiation. This paper examines epithelial wound healing in rabbit corneas with partial limbal deficiency (PLD), which was created by the surgical removal of two-thirds of the limbal zone (superior and inferior). Four to eight months after PLD creation, all corneas appeared normal, without vascularization. The residual stem cell capacity then was challenged by two sizes of corneal epithelial debridement created with combined n-heptanol and mechanical scraping. In the first group, two consecutive 6-mm defects were created 1 month apart. After the first wounding, three of eight PLD corneas had delayed wound healing and two of the three had vascularization, as compared to controls (n = 7). After the second wounding, both controls (n = 7) and the remaining PLD (n = 5) corneas showed similar rapid healing. In the second group, a large defect of up to 1 mm within the limbus was created. Healing was completed in 25-40 days in PLD (n = 6) corneas, a more marked delay compared to the 10-12 days for controls (n = 6) (P = 0.001). In addition, all PLD corneas showed increased vascularization and had epithelium of the conjunctival phenotype, verified by the immunofluorescent staining positive to AM-3 monoclonal antibody but negative to AE-5 monoclonal antibody. Thus, a deficiency of limbal stem cells contributes to the triad of conjunctival epithelial ingrowth, corneal vascularization, and delayed healing with recurrent erosion. In PLD, corneal epithelium is still compromised, particularly when a large epithelial cell mass is removed. |
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Authors:
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J J Chen; S C Tseng |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Investigative ophthalmology & visual science Volume: 31 ISSN: 0146-0404 ISO Abbreviation: Invest. Ophthalmol. Vis. Sci. Publication Date: 1990 Jul |
Date Detail:
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Created Date: 1990-08-13 Completed Date: 1990-08-13 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7703701 Medline TA: Invest Ophthalmol Vis Sci Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1301-14 Citation Subset: IM |
Affiliation:
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Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami School of Medicine, FL 33101. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Analysis of Variance Animals Antibodies, Monoclonal Conjunctiva / cytology Cornea / cytology* Disease Models, Animal Epithelial Cells Eye Burns / pathology Female Fluorescent Antibody Technique Male Neovascularization, Pathologic / pathology Phenotype Rabbits Sclera / cytology* Stem Cells / cytology Wound Healing* |
| Grant Support | |
ID/Acronym/Agency:
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EY-02180/EY/NEI NIH HHS; EY-06819/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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