Document Detail

Corepressor interaction differentiates the permissive and non-permissive retinoid X receptor heterodimers.
MedLine Citation:
PMID:  18282463     Owner:  NLM     Status:  MEDLINE    
Nurr1 is an orphan nuclear receptor regulating transcription both as a monomer and as a heterodimer with retinoid X receptor (RXR). RXR-Nurr1 heterodimers are permissive RXR heterodimers as they activate transcription in response to RXR ligands. In contrast, heterodimers formed by RXR and retinoic acid receptor (RAR) are non-permissive as they activate transcription only upon RAR ligand binding. We studied the mechanism mediating permissiveness and non-permissiveness by creating receptor chimeras between Nurr1 and RAR. We show that the amino-terminal part of the Nurr1 ligand binding domain conveys permissiveness to RXR-Nurr1 heterodimers. This region is involved in interactions with the corepressors SMRT and NcoR. The corepressors were released from RXR-Nurr1 heterodimers by RXR ligand binding. In contrast, RXR ligand increased the interaction between RXR-RAR heterodimers and the corepressors. The corepressors were released only upon binding of RAR ligand. In conclusion, corepressor interaction differentiates the permissive RXR-Nurr1 heterodimers from the non-permissive RXR-RAR heterodimers.
Johanna Lammi; Thomas Perlmann; Piia Aarnisalo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-02-08
Journal Detail:
Title:  Archives of biochemistry and biophysics     Volume:  472     ISSN:  1096-0384     ISO Abbreviation:  Arch. Biochem. Biophys.     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-03-31     Completed Date:  2008-04-28     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0372430     Medline TA:  Arch Biochem Biophys     Country:  United States    
Other Details:
Languages:  eng     Pagination:  105-14     Citation Subset:  IM    
Institute of Biomedicine/Physiology, Biomedicum Helsinki, University of Helsinki, P.O. Box 63, 00014 Helsinki, Finland.
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MeSH Terms
Cell Line
DNA-Binding Proteins / genetics,  physiology*
Nuclear Receptor Subfamily 4, Group A, Member 2
Protein Binding
Protein Structure, Tertiary
Receptors, Retinoic Acid / genetics,  physiology*
Recombinant Fusion Proteins / genetics,  metabolism
Retinoid X Receptors / genetics,  physiology*
Transcription Factors / genetics,  physiology*
Transcriptional Activation*
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Ligands; 0/NR4A2 protein, human; 0/Nuclear Receptor Subfamily 4, Group A, Member 2; 0/Receptors, Retinoic Acid; 0/Recombinant Fusion Proteins; 0/Retinoid X Receptors; 0/Transcription Factors

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