| Core exosome-independent roles for Rrp6 in cell cycle progression. | |
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MedLine Citation:
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PMID: 19225159 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Exosome complexes are 3' to 5' exoribonucleases composed of subunits that are critical for numerous distinct RNA metabolic (ribonucleometabolic) pathways. Several studies have implicated the exosome subunits Rrp6 and Dis3 in chromosome segregation and cell division but the functional relevance of these findings remains unclear. Here, we report that, in Drosophila melanogaster S2 tissue culture cells, dRrp6 is required for cell proliferation and error-free mitosis, but the core exosome subunit Rrp40 is not. Micorarray analysis of dRrp6-depleted cell reveals increased levels of cell cycle- and mitosis-related transcripts. Depletion of dRrp6 elicits a decrease in the frequency of mitotic cells and in the mitotic marker phospho-histone H3 (pH3), with a concomitant increase in defects in chromosome congression, separation, and segregation. Endogenous dRrp6 dynamically redistributes during mitosis, accumulating predominantly but not exclusively on the condensed chromosomes. In contrast, core subunits localize predominantly to MTs throughout cell division. Finally, dRrp6-depleted cells treated with microtubule poisons exhibit normal kinetochore recruitment of the spindle assembly checkpoint protein BubR1 without restoring pH3 levels, suggesting that these cells undergo premature chromosome condensation. Collectively, these data support the idea that dRrp6 has a core exosome-independent role in cell cycle and mitotic progression. |
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Authors:
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Amy C Graham; Daniel L Kiss; Erik D Andrulis |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2009-02-18 |
Journal Detail:
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Title: Molecular biology of the cell Volume: 20 ISSN: 1939-4586 ISO Abbreviation: Mol. Biol. Cell Publication Date: 2009 Apr |
Date Detail:
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Created Date: 2009-04-15 Completed Date: 2009-05-27 Revised Date: 2013-06-02 |
Medline Journal Info:
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Nlm Unique ID: 9201390 Medline TA: Mol Biol Cell Country: United States |
Other Details:
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Languages: eng Pagination: 2242-53 Citation Subset: IM |
Affiliation:
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Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis Cell Cycle* Cell Proliferation Chromosome Aberrations Chromosome Segregation Drosophila Proteins / deficiency, metabolism* Drosophila melanogaster / cytology*, genetics, metabolism* Exosomes / metabolism* Gene Expression Profiling Histones / metabolism Microtubules / metabolism Mitosis Mitotic Spindle Apparatus / metabolism Phenotype Phosphoproteins / metabolism Protein Binding Protein Subunits / metabolism Protein Transport |
| Grant Support | |
ID/Acronym/Agency:
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GM072820/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Drosophila Proteins; 0/Histones; 0/Phosphoproteins; 0/Protein Subunits |
| Comments/Corrections | |
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