Document Detail

Core-cross-linked alginate microcapsules for cell encapsulation.
MedLine Citation:
PMID:  19397289     Owner:  NLM     Status:  MEDLINE    
Self-cross-linkable polyelectrolyte pairs comprised of poly(methacrylic acid, sodium salt-co-2-[methacryloyloxy]ethyl acetoacetate) (70:30 mol ratio, A70) and poly-L-lysine are incorporated into CaAlg beads to form either a covalently cross-linked shell or a core-cross-linked bead. In both cases the reactive polyanion is added to a solution of sodium alginate that may contain live cells and dropped into a calcium chloride gelling bath. Subsequent exposure to poly-L-lysine (15-30 kDa) leads to formation of a cross-linked shell, while exposure to lower molecular weight poly-L-lysine (4-15 kDa) leads to formation of an interpenetrating matrix of covalently cross-linked synthetic polymer within the CaAlg template. The resulting spherical composites are resistant to chemical and mechanical stress yet remain cyto-compatible. This approach to cell-encapsulation may be useful for cell immuno-isolation in therapeutic cell transplants.
M A Jafar Mazumder; Nicholas A D Burke; Feng Shen; Murray A Potter; Harald D H Stöver
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biomacromolecules     Volume:  10     ISSN:  1526-4602     ISO Abbreviation:  Biomacromolecules     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-06-08     Completed Date:  2009-10-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100892849     Medline TA:  Biomacromolecules     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1365-73     Citation Subset:  IM    
Department of Chemistry, McMaster University, Hamilton, Ontario, Canada.
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MeSH Terms
Alginates / chemistry*
Cell Line
Glucuronic Acid / chemistry
Hexuronic Acids / chemistry
Polymers / chemistry
Rhodamines / chemistry
Reg. No./Substance:
0/Alginates; 0/Capsules; 0/Hexuronic Acids; 0/Polymers; 0/Rhodamines; 576-37-4/Glucuronic Acid; 9005-32-7/alginic acid

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