| Core-cross-linked alginate microcapsules for cell encapsulation. | |
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MedLine Citation:
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PMID: 19397289 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Self-cross-linkable polyelectrolyte pairs comprised of poly(methacrylic acid, sodium salt-co-2-[methacryloyloxy]ethyl acetoacetate) (70:30 mol ratio, A70) and poly-L-lysine are incorporated into CaAlg beads to form either a covalently cross-linked shell or a core-cross-linked bead. In both cases the reactive polyanion is added to a solution of sodium alginate that may contain live cells and dropped into a calcium chloride gelling bath. Subsequent exposure to poly-L-lysine (15-30 kDa) leads to formation of a cross-linked shell, while exposure to lower molecular weight poly-L-lysine (4-15 kDa) leads to formation of an interpenetrating matrix of covalently cross-linked synthetic polymer within the CaAlg template. The resulting spherical composites are resistant to chemical and mechanical stress yet remain cyto-compatible. This approach to cell-encapsulation may be useful for cell immuno-isolation in therapeutic cell transplants. |
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Authors:
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M A Jafar Mazumder; Nicholas A D Burke; Feng Shen; Murray A Potter; Harald D H Stöver |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biomacromolecules Volume: 10 ISSN: 1526-4602 ISO Abbreviation: Biomacromolecules Publication Date: 2009 Jun |
Date Detail:
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Created Date: 2009-06-08 Completed Date: 2009-10-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100892849 Medline TA: Biomacromolecules Country: United States |
Other Details:
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Languages: eng Pagination: 1365-73 Citation Subset: IM |
Affiliation:
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Department of Chemistry, McMaster University, Hamilton, Ontario, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alginates
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chemistry* Animals Capsules* Cell Line Glucuronic Acid / chemistry Hexuronic Acids / chemistry Mice Permeability Polymers / chemistry Rhodamines / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Alginates; 0/Capsules; 0/Hexuronic Acids; 0/Polymers; 0/Rhodamines; 576-37-4/Glucuronic Acid; 9005-32-7/alginic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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