| Cordyceps pruinosa extracts induce apoptosis of HeLa cells by a caspase dependent pathway. | |
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MedLine Citation:
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PMID: 20138133 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM OF THE STUDY: Cordyceps is a parasitic fungus and has long been used as a traditional Chinese medicine to treat illnesses, promote longevity, increase athletic power, and relieve exhaustion and cancer. In this study, we reveal the mechanisms underlying apoptosis induced by Cordyceps pruinosa butanol fraction (CPBF) in the human cervical adenocarcinoma cell line, HeLa. MATERIALS AND METHODS: Proliferation and apoptosis of cells were examined by MTT assay, DNA fragmentation, phosphatidyl serine distribution assay, Western blot analysis, and immunocytochemistry. To determine the association between CPBF related apoptosis and ROS, electron spin resonance (ESR) trapping experiments were used. RESULTS: CPBF inhibited proliferation and induced apoptosis in HeLa cells in a dose-dependent manner using a MTT assay, DNA fragmentation, and a phosphatidyl serine distribution assay. Western blot analysis showed that apoptosis in HeLa cells was caspase-3- and -9-dependent. Proteolytic cleavage of PARP and the release of cytochrome c from the mitochondria into the cytosol were significantly increased and the Bcl-2/Bax protein ratio was decreased. Apoptosis induced by CPBF was not prevented by various antioxidants. CONCLUSIONS: These results indicate that apoptotic effects of CPBF on HeLa cells are mediated by mitochondria-dependent death-signaling pathway independent of reactive oxygen species, suggesting that CPBF might be effective as an anti-proliferative agent for cancer. |
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Authors:
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Ho Gyoung Kim; Heesang Song; Deok Hyo Yoon; Byeong-Wook Song; Sang Min Park; Gi Ho Sung; Jae-Youl Cho; Hae Il Park; Sunga Choi; Won O Song; Ki-Chul Hwang; Tae Woong Kim |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-02-04 |
Journal Detail:
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Title: Journal of ethnopharmacology Volume: 128 ISSN: 1872-7573 ISO Abbreviation: J Ethnopharmacol Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-03-09 Completed Date: 2010-07-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7903310 Medline TA: J Ethnopharmacol Country: Ireland |
Other Details:
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Languages: eng Pagination: 342-51 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2010 Elsevier Ireland Ltd. All rights reserved. |
Affiliation:
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Department of Biochemistry, Kangwon National University, Chuncheon 200-701, Republic of Korea. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects* Caspase 3 / metabolism* Caspases / metabolism* Cordyceps / metabolism* Cytochrome c Group / metabolism Cytochromes c / metabolism DNA Fragmentation / drug effects Hela Cells Humans Mitochondria / metabolism Plant Extracts / pharmacology* Poly(ADP-ribose) Polymerases / metabolism Reactive Oxygen Species / metabolism Signal Transduction / drug effects bcl-2-Associated X Protein / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Cytochrome c Group; 0/Plant Extracts; 0/Reactive Oxygen Species; 0/bcl-2-Associated X Protein; 116110-46-4/cytochrome c''; 9007-43-6/Cytochromes c; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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