Document Detail

Cord blood red cell osmotic fragility: a comparison between preterm and full-term newborn infants.
MedLine Citation:
PMID:  12706310     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: The osmotic fragility of red blood cells reflects their membrane ability to maintain structural integrity. The osmolality at which the cells lyse is related to their shape, deformability, surface area/volume ratio and intrinsic membrane properties. In cord blood, there may be differences between premature and term infants, and be influenced by maternal medication and other factors. There have been no definitive findings on possible differences between preterm and full-term infant osmotic fragility. AIMS: To determine if cord blood erythrocyte osmotic fragility differs between premature and full-term newborn infants, using two parallel techniques. PATIENTS AND METHODS: Cord blood samples were obtained from preterm singletons (N=11), preterm multiple births (N=10), full-term infants (N=24), as well as adults (N=22), for comparison. An osmotic fragility test was used to determine the NaCl concentration at which 20%, 50% and 80% of hemolysis occurred using individual logistic curves. A glycerol lysis test determined the time needed to lyse 50% of red blood cells. RESULTS: Cord blood red cells of multiple birth premature infants were more hemolysis-resistant than erythrocytes from full-term infants or adults. Another index of osmotic fragility, the difference in NaCl concentration for 80% and 20% red cell hemolysis showed that premature infants had greater differences than full-term infants or adults. Glycerol lysis time revealed that both preterm and full-term infants had an erythrocyte subpopulation that took longer than adult blood to attain 50% hemolysis. Correlation between both tests was very significant (r=-0.603, P<0.0001, N=67). CONCLUSIONS: This study shows that erythrocytes of premature infants, although, in average, less osmotically fragile than those of healthy full-term infants, contain a more hemolysis-susceptible cell subpopulation.
Maria L G Bautista; Waseem Altaf; Ritu Lall; Raul A Wapnir
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Early human development     Volume:  72     ISSN:  0378-3782     ISO Abbreviation:  Early Hum. Dev.     Publication Date:  2003 May 
Date Detail:
Created Date:  2003-04-22     Completed Date:  2003-10-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7708381     Medline TA:  Early Hum Dev     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  37-46     Citation Subset:  IM    
Division of Neonatal/Perinatal Medicine, Schneider Children's Hospital at North Shore, North Shore-Long Island Jewish Health System, 300 Community Drive, Manhasset, NY 11030, USA.
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MeSH Terms
Erythrocytes / physiology*
Fetal Blood / cytology*
Glycerol / pharmacology
Hemolysis / physiology
Infant, Newborn / blood*
Infant, Premature / blood*
Middle Aged
Osmotic Fragility
Pregnancy, Multiple
Sodium Chloride / pharmacology
Statistics, Nonparametric
Reg. No./Substance:
56-81-5/Glycerol; 7647-14-5/Sodium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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