| Cord blood vitamin D status impacts innate immune responses. | |
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MedLine Citation:
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PMID: 21470993 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: Our objectives were to 1) assess cord blood vitamin D concentrations from healthy term newborns, 2) ascertain whether cord blood vitamin D insufficiency precludes optimal induction of the Toll-like receptor (TLR) antimicrobial pathway in monocytes, and 3) determine whether in vitro supplementation with 25-hydroxyvitamin D(3) [25(OH)D(3)] and/or 1,25-dihydroxyvitamin D(3) [1,25(OH)(2)D(3)] restores TLR-induced antimicrobial responses. STUDY DESIGN: Plasma concentrations of 25(OH)D and 1,25(OH)(2)D were measured from cord blood of 23 newborns. Human monocytes were cultured in cord blood plasma and stimulated with TLR2 and TLR4 ligands, and then antimicrobial gene expression was analyzed using quantitative PCR. RESULTS: Cord blood 25(OH)D and 1,25(OH)(2)D concentrations were positively correlated to each other (r = 0.78; P <0.0001). Compared with those conditioned in vitamin D-sufficient plasma [25(OH)D > 75 nmol/liter], monocytes cultured in severely vitamin D-deficient plasma [25(OH)D < 30 nmol/liter] exhibited decreased TLR-induced cathelicidin expression (P <0.05). Supplementation in vitro of vitamin D-deficient plasma with 25(OH)D(3) increased antimicrobial peptide gene expression. CONCLUSIONS: Cord blood vitamin D deficiency, by its effects on TLR-induced antimicrobial production, altered in vitro monocyte responses. The observation that exogenous 25(OH)D(3) in vitro recovered TLR-induced antimicrobial responses suggests the need for additional prospective investigations to further delineate the role of vitamin D in the newborn immune response. |
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Authors:
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Valencia P Walker; Xiaoran Zhang; Ida Rastegar; Philip T Liu; Bruce W Hollis; John S Adams; Robert L Modlin |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-04-06 |
Journal Detail:
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Title: The Journal of clinical endocrinology and metabolism Volume: 96 ISSN: 1945-7197 ISO Abbreviation: J. Clin. Endocrinol. Metab. Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-05-23 Completed Date: 2011-08-05 Revised Date: 2012-05-11 |
Medline Journal Info:
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Nlm Unique ID: 0375362 Medline TA: J Clin Endocrinol Metab Country: United States |
Other Details:
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Languages: eng Pagination: 1835-43 Citation Subset: AIM; IM |
Affiliation:
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Department of Pediatrics, Division of Neonatology and Developmental Biology, David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, California 90095, USA. vpwalker@mednet.ucla.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aged Cells, Cultured Female Fetal Blood / immunology*, metabolism Gene Expression Humans Immunity, Innate / genetics, immunology* Infant, Newborn Male Middle Aged Monocytes / cytology, immunology, metabolism Reverse Transcriptase Polymerase Chain Reaction Toll-Like Receptor 2 / genetics, immunology, metabolism Toll-Like Receptor 4 / genetics, immunology, metabolism Vitamin D / blood*, immunology Vitamin D Deficiency / blood, immunology* |
| Grant Support | |
ID/Acronym/Agency:
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A147868//PHS HHS; A173539//PHS HHS; R01 AI047868/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Toll-Like Receptor 2; 0/Toll-Like Receptor 4; 1406-16-2/Vitamin D |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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