Document Detail


Coral and macroalgal exudates vary in neutral sugar composition and differentially enrich reef bacterioplankton lineages.
MedLine Citation:
PMID:  23303369     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Increasing algal cover on tropical reefs worldwide may be maintained through feedbacks whereby algae outcompete coral by altering microbial activity. We hypothesized that algae and coral release compositionally distinct exudates that differentially alter bacterioplankton growth and community structure. We collected exudates from the dominant hermatypic coral holobiont Porites spp. and three dominant macroalgae (one each Ochrophyta, Rhodophyta and Chlorophyta) from reefs of Mo'orea, French Polynesia. We characterized exudates by measuring dissolved organic carbon (DOC) and fractional dissolved combined neutral sugars (DCNSs) and subsequently tracked bacterioplankton responses to each exudate over 48 h, assessing cellular growth, DOC/DCNS utilization and changes in taxonomic composition (via 16S rRNA amplicon pyrosequencing). Fleshy macroalgal exudates were enriched in the DCNS components fucose (Ochrophyta) and galactose (Rhodophyta); coral and calcareous algal exudates were enriched in total DCNS but in the same component proportions as ambient seawater. Rates of bacterioplankton growth and DOC utilization were significantly higher in algal exudate treatments than in coral exudate and control incubations with each community selectively removing different DCNS components. Coral exudates engendered the smallest shift in overall bacterioplankton community structure, maintained high diversity and enriched taxa from Alphaproteobacteria lineages containing cultured representatives with relatively few virulence factors (VFs) (Hyphomonadaceae and Erythrobacteraceae). In contrast, macroalgal exudates selected for less diverse communities heavily enriched in copiotrophic Gammaproteobacteria lineages containing cultured pathogens with increased VFs (Vibrionaceae and Pseudoalteromonadaceae). Our results demonstrate that algal exudates are enriched in DCNS components, foster rapid growth of bacterioplankton and select for bacterial populations with more potential VFs than coral exudates.
Authors:
Craig E Nelson; Stuart J Goldberg; Linda Wegley Kelly; Andreas F Haas; Jennifer E Smith; Forest Rohwer; Craig A Carlson
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2013-01-10
Journal Detail:
Title:  The ISME journal     Volume:  7     ISSN:  1751-7370     ISO Abbreviation:  ISME J     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-04-24     Completed Date:  2013-12-04     Revised Date:  2014-05-07    
Medline Journal Info:
Nlm Unique ID:  101301086     Medline TA:  ISME J     Country:  England    
Other Details:
Languages:  eng     Pagination:  962-79     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Anthozoa / chemistry,  metabolism,  microbiology*
Bacteria / classification*,  growth & development,  isolation & purification,  metabolism
Carbohydrate Metabolism
Carbon / metabolism
Chlorophyta / chemistry,  microbiology
Coral Reefs
Hydrogen-Ion Concentration
Plankton / classification,  growth & development,  isolation & purification,  metabolism
Polynesia
RNA, Ribosomal, 16S
Rhodophyta / chemistry,  microbiology
Seawater / chemistry*,  microbiology*
Seaweed / chemistry,  microbiology*
Chemical
Reg. No./Substance:
0/RNA, Ribosomal, 16S; 7440-44-0/Carbon
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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