| Copper(II) complexes with N,N'-dialkyl-1,10-phenanthroline-2,9-dimethanamine: synthesis, characterization, DNA-binding thermodynamical and kinetic studies. | |
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MedLine Citation:
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PMID: 11597465 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Copper(II) complexes (Cu-L, L=N,N'-dialkyl-1,10-phenanthroline-2,9-dimethanamine) were synthesized and characterized by elemental analyses, IR spectra and conductance measurement. The interaction of the copper(II) complex with calf thymus DNA was studied by means of UV melting experiments, fluorescence spectra and circular dichroic spectra. Using ethidium bromide as a fluorescence probe, the binding mode of the complexes Cu-L with calf-thymus DNA was studied spectroscopically. The results indicate that the complexes Cu-L perhaps interact with calf-thymus DNA by both intercalative and covalent binding. Kinetics of binding of the cupric complexes to DNA was studied for the first time using ethidium bromide as a fluorescence probe with stopped-flow spectrophotometer under pseudo-first-order condition. The stronger binding of two steps in the process of the complexes Cu-L interacting with DNA was observed, and the probable interaction process was discussed in detail. The corresponding k(obs) and E(a) of binding to DNA (where k(obs) is the observed pseudo-first-order rate constant, E(a) is the observed energy of activation) were obtained. |
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Authors:
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Z M Wang; H K Lin; Z F Zhou; M Xu; T F Liu; S R Zhu; Y T Chen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Bioorganic & medicinal chemistry Volume: 9 ISSN: 0968-0896 ISO Abbreviation: Bioorg. Med. Chem. Publication Date: 2001 Nov |
Date Detail:
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Created Date: 2001-10-12 Completed Date: 2002-03-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9413298 Medline TA: Bioorg Med Chem Country: England |
Other Details:
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Languages: eng Pagination: 2849-55 Citation Subset: IM |
Affiliation:
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Department of Chemistry, Nankai University, 300071, Tianjin, PR China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amines
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chemistry Animals Binding Sites Circular Dichroism Copper / chemistry* DNA / chemistry, metabolism* Kinetics Nucleic Acid Conformation / drug effects Organometallic Compounds / chemistry, metabolism* Phenanthrolines / chemistry Spectrometry, Fluorescence Temperature Thermodynamics |
| Chemical | |
Reg. No./Substance:
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0/Amines; 0/Organometallic Compounds; 0/Phenanthrolines; 7440-50-8/Copper; 9007-49-2/DNA; 91080-16-9/calf thymus DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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