Document Detail

Copper transporter 1, metallothionein and glutathione reductase genes are differentially expressed in tissues of sea bream (Sparus aurata) after exposure to dietary or waterborne copper.
MedLine Citation:
PMID:  18304880     Owner:  NLM     Status:  MEDLINE    
The high affinity copper transporter 1 (Ctr1), metallothionein (MT) and glutathione reductase (GR) are essential for copper uptake, sequestration and defense respectively. Following rearing on a normal commercial diet (12.6+/-0.2 mg kg(-1) Cu), sea bream were fed an experimental control diet lacking mineral mix (7.7+/-0.3 mg kg(-1) Cu), an experimental diet enhanced with Cu (135+/-4 mg kg(-1) Cu) or an experimental diet (7.7+/-0.3 mg kg(-1) Cu) whilst exposed to Cu in water (0.294+/-0.013 mg L(-1)). Fish were sampled at 0, 15 and 30 days after exposures. Fish fed the Cu-enhanced experimental diet showed lower levels of expression of Ctr1 in the intestine and liver compared to fish fed control experimental diets, whilst Ctr1 expression in the gill and kidney was unaffected by excess dietary Cu exposure. Waterborne-Cu exposure increased Ctr1 mRNA levels in the intestine and the kidney compared to experimental controls. Excess dietary Cu exposure had no effect on levels of metallothionein (MT) mRNA, and the only effect of dietary excess Cu on glutathione reductase (GR) mRNA was a decrease in the intestine. Both MT mRNA and GR were increased in the liver and gill after waterborne-Cu exposure, compared to levels in fish fed experimental control low Cu diets. Thus, Ctr1, MT and GR mRNA expression in response to excess Cu is dependent on the route of exposure. Furthermore, the tissue expression profile of sea bream Ctr1 is consistent with the known physiology of copper exposure in fish and indicates a role both in essential copper uptake and in avoidance of excess dietary and waterborne copper influx.
M Minghetti; M J Leaver; E Carpenè; S G George
Publication Detail:
Type:  Journal Article     Date:  2008-02-07
Journal Detail:
Title:  Comparative biochemistry and physiology. Toxicology & pharmacology : CBP     Volume:  147     ISSN:  1532-0456     ISO Abbreviation:  Comp. Biochem. Physiol. C Toxicol. Pharmacol.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-04-08     Completed Date:  2008-06-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100959500     Medline TA:  Comp Biochem Physiol C Toxicol Pharmacol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  450-9     Citation Subset:  IM    
Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, United Kingdom.
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MeSH Terms
Amino Acid Sequence
Cation Transport Proteins / genetics*,  metabolism
Copper / administration & dosage,  toxicity*
Gene Expression / drug effects*
Gills / drug effects,  metabolism
Glutathione Reductase / genetics*,  metabolism
Intestines / drug effects,  metabolism
Liver / drug effects,  metabolism
Metallothionein / genetics*,  metabolism
Molecular Sequence Data
Oligonucleotide Array Sequence Analysis
RNA, Messenger / metabolism
Sea Bream*
Sequence Analysis, Protein
Reg. No./Substance:
0/Cation Transport Proteins; 0/RNA, Messenger; 0/copper transporter 1; 7440-50-8/Copper; 9038-94-2/Metallothionein; EC Reductase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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